Definite Focus		Details
	New feature designed to eliminate Z drift, thus ensuring maximum reliability in live cell imaging. enlarge Keep focused. Definite Focus uses the infrared light of an LED to sense the path length between the objective and the bottom of the culture vessel, and corrects the focusing, if necessary, via the Z control of Axio Observer.Z1. You can start experiments without waiting for the complete microscope to reach thermal stability. Unrestricted fluorescence microscopy. The wavelength of the LED is 835 nm – way out of the excitation and emission spectra of all common fluorochromes. Definite Focus permits dynamic temperature experiments. The desired temperature jumps are no problem, as Definite Focus will immediately compensate any drift. Unimpaired flexibility. Definite Focus is compatible with all customary objectives and contrast methods. Use dishes with plastic or glass bottoms, as your application requires. Use the unique operating concept of Axio Observer.Z1: The central TFT display lets you control or watch Definite Focus just like all other microscope components. Operating principle		Axio Observer More Security Definite Focus Incubation Concept Incubators Pyramid Design Aqua Stop II Article G.I.T. 03/2008 „Always in Focus“, Imaging & Microscopy ,
	Definite Focus projects a grid onto the vessel bottom, which reflects it onto a sensor chip. Since the chip is inclined, only a narrow zone of the grid is sharply defined. It is kept in focus by the Z control of Axio Observer to compensate any drifts.
	enlarge The beam combiner
	The beam combiner for the Definite Focus feature is located inside the revolving objective nosepiece. If necessary, it can quickly be removed and replaced with other items for special applications.