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Improve your FRET and pH imaging experiments with the advantage of syncronized and simultaneous image capture.
The new developed Dual Camera option for AxioVision allows you to expand the Fast Acquisition and Physiology modules.
Using two cameras in parallel offers important advantages: you can acquire images in two channels at the same time. This feature is especially beneficial when capturing time-sensitive experiments, like FRET, pH or Calcium imaging. Image pairs synchronized and are no longer temporally delayed. The potential image shift between the channels can no longer occur.
Also combinations of various contrasting methods in transmitted-light and fluorescence microscopy are possible with AxioVision Dual Camera. This module is therefore indispensable for your actual and future electrophysiological or neurobiological applications.
How to acquire multi labelled beads with the Dual Camera module is shown in the movie.
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The time lapse Z-stack image shows HeLa cells with YFP-labeled endoplasmic reticulum and mTFP1-labeled mitochondria. The images were acquired with Cell Observer HS, using the 63x Plan APOCHROMAT objective and two synchronized AxioCam MRm cameras.
The following modules were used: Multichannel Fluorescence, Time Lapse, Z-Stack, Fast Acquisition, Dual Camera, Widefield Multichannel Unmixing, 3D Deconvolution
Images reproduced with the kind permission of Christian Junker, working group of Prof. Hoth, Saarland University Faculty of Medicine, Homburg, Germany. |
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 Use additional cameras
With the AxioVision Release 4.8 you can - besides the products of the AxioCam series - also use cameras based on the Photometrics PVCAM standard. The module thereby also supports the EMCCD-Model Evolve.
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