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Line scanners are a form of parallel point scanning systems like Spinning Disk microscopes. Instead of projecting hundreds of spots, line scanners project a single line of illumination into the sample which is swept across the sample.
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| Zebrafish embryo: Fluorescence of Erythrocytes (dsRed : Red) and endothelial cells (eGFP : Green) is captured simultaneously in 2 channels at 33 frames / second. Specimen: S. Hermanson and S. C. Ekke, University of Minnesota, USA. |
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The returning emission is filtered through a confocal slit aperture to reject out-of-focus light before being detected on a line detector. The line scanners are more complex than the Spinning Disk systems, but offer slightly better discrimination of out-of-focus light and additional flexibility in scan modes. For medium to low image resolution, line scanners offer the highest
possible frame rate.
Current product:
LSM 7 LIVE
Legacy product:
LSM 5 LIVE | |
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