Applications for Light Microscopy - BioMed
Laser TIRF 3 - Laser Wavelengths
New possibilities: more lasers for Multi-color TIRF
With the new laser module in Laser TIRF 3, up to six laser
wavelengths can be selected. Therewith even more
fluorophores can be optimally excited if more than one
fluorescent marker is used.

Examples of dyes and markers for the available
wavelenghts

Laser lines405 458 488 514 532561 635
BFP
(Blue Fluorescent Protein)
x
CFP
(Cyan Fluorescent Protein)
x
x
FITC
x
ECFP (Enhanced Cyan Fluorescent Protein)
x
GFP (S65T)
x
x
wtGFP
x
x
EGFP (Enhanced Green Fluorescent Protein)
x
x
YFP
x
EYFP (Enhanced Yellow Fluorescent Protein)
x
DsRed
x
x
mRFP
x
x
HcRed
x
x
mRaspberry
x
mPlum pH 7
x
mPlum pH 11
x
Texas Red-X conjugate
x
Cy2
x
x
Cy3
x
x
Cy3.5
x
x
Cy5
x
Cy5.5
x
Alexa 405
x
Alexa 430
x
x
Alexa 488
x
Alexa 532
x
Alexa 546
x
Alexa 555
x
x
Alexa 568
x
Alexa 594
x
x
Alexa 633
x
x
Alexa 647
x
Alexa 660
x
DiI
x
x
DiO
x
x
Details
Laser TIRF 3

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