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In an aperture correlation system, the excitation light is modulated using a grid structure on a Spinning Disk in a conjugate plane. Returning fluorescence from the focal plane passes through the grid structure and forms an image which in addition to the information from the focal plane contains some out-of-focus contributions which also pass through the grid.
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LLC-PK1 cells, Tubulin Emerald (green), H2B-mCherry (red),
63x/1.4 Plan APOCHROMAT. |
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Light reflected off the disk forms an image with only out-of-focus contributions. From these images both the widefield and the optically sectioned image can be derived by a simple calculation. Aperture Correlation offers the light efficiency and out-of-focus discrimination of Structured Illumination but enables faster acquisition.
Current product:
VivaTome | |
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