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Excellent Results Even with Extremely Weak Signals.
IC²S stands for Infinity Contrast & Color Corrected System. This patented beam path is based on the optimization of the proven Carl Zeiss ICS Infinity Optics (ICS). New: the transmitted light beam path for uniform illumination. The optics of the universal and long-distance condensers have been adapted to all applications. Even at low magnifications and large working distances considerably better resolution and contrast is achieved.
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| Nosepiece with DIC objectives |
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Unrivalled in every respect: the objectives
For the new Axio Imager 2 product line Carl Zeiss has extended the high-performance objectives especially for high magnifications, for both fixed and live cell imaging applications.
- The EC Plan-NEOFLUAR universal objectives. The consistent stray light minimization results in a definite contrast enhancement, which is critical in all microscopic techniques.
- The Plan-APOCHROMAT objectives convince through their outstanding point spread function and their unparalleled planar and chromatic correction.
- The αPlan-APOCHROMAT objectives 100x/1.46 Oil and 100x/1.57 HI Oil * provide maximum resolution in fluorescence and transmitted light DIC techniques because of their high numerical apertures.
*available from Fall 2009
- The LCI Plan-NEOFLUAR objectives 25x/0.8 and 63x/1.3 Imm. corr. were conceived for live cell Imaging techniques and calibrated for specific temperature intervals as well as immersion media from water to glycerin.
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| LED – the new light source for Köhler illumination | LED for Fixed Köhler illumination |
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Constant color temperature: the LED illumination sources
The interesting alternative to conventional halogen illumination with compelling advantages: constant color temperature independent of the brightness, low heat radiation and long service life.
LED illumination also has a filter mount for individual setting of the color temperature. For the first time such an illumination source is also offered with a trigger input for high frequency switching. For more simple applications there is a variant available which is attached directly beneath the condenser. In accordance with the Fixed Köhler Principle, for simple adjustment with all contrast techniques.
See more: DIC or DIC + Fluorescence
Optimized DIC for the new generation Axio Imager 2: uniform interference contrast at all magnifications from 5x to 100x across the entire field of view. Particularly in digital imaging the shading correction becomes obsolete. You always have a uniformly illuminated DIC image.
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Olfactory bulb (frog),
image taken with DIC.
D. Schild, Univ. Göttingen, Germany | Olfactory bulb (frog), multichannel fluorescence with ApoTome.
Green: projections of olfactory sensory cells. Red: cell nuclei. |
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For the first time these advantages are now also reproducible and can be adjusted via motorized control. With the new motorized DIC turret for transmitted light DIC you can now automatically shift between high-resolution and high-contrast interference contrast. The contrast settings can be separately stored for each user and for each magnification used. You can also combine DIC imaging with fluorescence excitation extremely simple and automatically. Without sample-induced artifacts.
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| Motorized DIC turret for reproducible contrast adjustment |
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