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Widefield fluorescence microscopes acquire both the in-focus and the out-of-focus light contributions from the sample. This makes them one of the most sensitive systems for live cell imaging because no emitted light is thrown away.
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| Four dictyostelium cells shortly after spore germination in the vegetative stage, stably transfected with a GFP construct. The pseudopodia growth that is typical for this stage is clearly visible. Sample: Ralph Gräf, University of Potsdam, Germany. |
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The majority of the out-of-focus light is derived from the point-spread function (PSF). A precise model for the PSF can be used to deconvolve image stacks. It is both a very light-efficient and cost-effective approach to generate 3D data. Deconvolution can also be used to enhance other optical sectioning techniques.
Current product:
Cell Observer | |
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