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Conventional dyes have a broad excitation spectrum when excited with pulsed NIR lasers. The excitation spectra of different dyes may largely overlap.
The table provides an overview of the excitation spectra obtained with different dyes. The data are based on empirical measurements; they may slightly vary with specimen treatment. (See also: Xu, et al. (1996), PNAS, 93, 10763)
| Fluorochrome | Absorption | Emission |
| Alexa Fluor 350 | 720-800 | 440 |
| Alexa Fluor 488 | 720-800 | 515 |
| Alexa Fluor 546 | 720-840 | 569 |
| Alexa Fluor 568 | 720-840 | 596 |
| Alexa Fluor 594 | 720-850 | 610 |
| Alexa Fluor 633 | 720-900 | 647 |
| AMCA | 780-800 | 444 |
| bis-MSB | 680-750 | 420 |
| Bodipy | 900-950 | 512 |
| Calcium Crimson | 900 | 615 |
| Calcium green | 780-850 | 531 |
| Cascade Blue | 750-800 | 420 |
| Coumarin 307 | 780-800 | 530 |
| CY2 | 780-800 | 506 |
| CY3 | 780 | 565, 615 |
| CY5 | 780-820 | 670 |
| Dansyl Hydrazine | 700-750 | 440 |
| DAPI, Hoechst | 700-820 | 455, 478 |
| DiA | 800-860 | 580 |
| DID | 780-820 | 670 |
| DiO | 780-830 | 510 |
| eCFP | 800-900 | 476 |
| eGFP | 820-950 | 509 |
| eYFP | 860-950 | 532 |
| Fluorescein | 780 - 820 | 519 |
| Indo-1 free | 690-720 | 490 |
| Indo-1 Ca2+ | 690-720 | 400 |
| Lucifer Yellow | 860-890 | 533 |
| Mito Tracker red | 750-840 | 600 |
| Nile Red | 810 | 640 |
| Oregon Green | 780-860 | 526 |
| Propidium Iodide (PI) | 820-850 | 617 |
| Rhodamin B | 800-840 | 600 |
| Rhodamine 123 | 780-860 | 550 |
| Sytox Green | 740-760 or 880-940 | 524 |
| TRITC | 800-840 | 572 |
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