MultiChannel Unmixing
Crosstalk removal by Automatic Component Extraction
Overview
Multifluorescence
Emission Fingerprinting
Excitation Fingerprinting
MultiChannel Unmixing
Multitracking
Online Fingerprinting
Automatic Component Extraction
Solving the problem of exitation crosstalk
Unmixing for tricky dual labelling experiments
appropriate products
LSM 7
LIVE
LSM 710
LSM 710 NLO
LSM 700
LSM 710 ConfoCor 3
Deutsche Version
MultiChannel Unmixing is a function for the removal of crosstalk between fluorescent dyes in channel based LSM systems from Carl Zeiss.
While it was not possible to remove excitation crosstalk deriving from simultaneous excitation of dyes so far, it is now possible to use the Zeiss Automatic Component Extraction (ACE) also on single detector based LSM systems. The results are clearly separated images even with fluorescent proteins and other demanding dye combinations.
Left: strong channel crosstalk; right clear separation with MultiChannel Unmixing.
Top: Nerve cells, labelled with Cy3 and EGFP; simultaneous excitation with 488 and 543nm and imaged in two channels 505-530nm and 560-615nm. Bottom: Mouse kidney, Alexa dye combination simultaneously excited with 488 and 543nm and imaged in two channels 505-530nm and 560-615nm.