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Beam path in the confocal laser scanning microscope. |
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Energy diagram of fluorescence excitation,
single photon excitation (left), multiphoton excitation (right). |
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Excitation efficiency and emission intensity as a function of excitation wavelength. |
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Separation of fluorescence emissions by means of dichroic filters. |
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Beam path inside the scanning Module (schematic). |
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Calcium imaging using the FRET indicator Yellow Cameleon 2. |
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