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| * all images with kind permission of the authors |
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Multi-photon lifetime imaging of fluorescent proteins
Multi-photon excited fluorescence lifetime images of cultured cells transfected with a GFP-tagged protein (a), YFP-tagged protein (b) and both GFP- and YFP- tagged proteins (c), (d). The colour bars show the calibration of fluorescence lifetime from approx 2.1 ns (red) to 3.0 ns (dark blue). Although their fluorescence emissions overlap significantly in the green portion of the spectrum, the GFP tags show a significantly shorter lifetime than the YFP tags in the single labelled cells and can be distinguished in the co-transfected cells. Separation of these probes can be further improved by correcting for the instrument response of around 0.2 - 0.3.
Nick White and Rachel Errington, Oxford University, UK
 Overview
Gallery 1 | 2 |