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| Contact-free manipulation and microdissection with lasers - the principle |
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In PALM systems, separation of the specimen occurs with a laser beam focused so precisely that a beam accuracy of less than 1 µm can be achieved.
The selected specimen is removed with a software-controlled laser beam and catapulted by a laser pulse into a collection device. This procedure ensures that no unwanted elements reach the specimen. Because the laser in the process is only directed at the sample for about 1 ns, it does not transfer any heat. The process is completely contact and contamination-free and guarantees the best possible preservation of the material.
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Literature
Verena Horneffer, Norbert Linz, Alfred Vogel: Principles of laser-induced separation and transport of living cells, Journal of Biomedical Optics,
12 (5), 054016 September/October 2007 |
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