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| Example 1: GFP and YFP | |
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| Human epidermoid tumor cells A431 expressing GFP and a YFP-Rab11 fusion protein. The image shows a Lambda Stack of 16 spectrally resolved images using the gallery view mode of the LSM 510 META. | |  Emission Fingerprinting |
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| Emission Fingerprinting visualizes diffuse GFP distribution throughout one of the cells shown and punctate (vesicular) YFP labeling in both cells. | | |
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| J. Rink, Max-Planck-Institute of Cell Biology and Genetics, Dresden, Germany |
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| Example 2: CFP, GFP and YFP | | |
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| Living cultured cell expressing GFP and a YFP-phosphatase fusion protein - result of Emission Fingerprinting. | | |
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| Dr. F. Boehmer, Friedrich-Schiller-University, Jena, Germany |
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| Example 3: CFP, GFP and YFP | | |
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 | Mixture of NIH3T3 cells expressing either CFP-, GFP- or YFP-histone 2B fusion proteins.
Despite their widely overlapping emission spectra nuclear fluorescence emissions of the different types of fluorescent proteins are clearly separated by Emission Fingerprinting. |
| Dr. R. Lansford, Dr. M. Dickinson, Prof. S. Fraser, Caltech, Pasadena, USA |
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| Example 4: GFP and FITC | | |
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| Cultured cells expressing ECFP-RanGAP1 (blue), EGFP-emerin (green) and EYFP-SUMO1 fusion proteins (red). Emission Fingerprinting demonstrates localization of the proteins to the cellular compartments cytosol, endoplasmic reticulum / nuclear membrane and nucleus, respectively. | | |
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 | Prof. Y. Hiraoka, KARC, Kobe, Japan |
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| Example 5: FITC und GFP | | |
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| Cultured fibroblasts expressing a GFP-Histone2B fusion protein. Actin filaments of the sample were stained with FITC-phalloidin. Both fluorecent markers show a distance of emission peaks of about 7nm.
The left image depicts a Lambda Coded representation of the Lambda Stack, where pixel colors are assigned with respect to the wavelength of maximum emission intensity. The graph shows emission spectra of GFP and FITC directly determined in the sample using the Mean-of-ROI function of the LSM 510 META. | | |
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| The result of Emission Fingerprinting: Crosstralk-free separation of signals despite heavy spectral and spatial overlap of emission spectra. |
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| Dr. M. Dickinson, Caltech, Pasadena, USA |
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