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| | The specimen is illuminated with natural, i.e. non-polarized light. Incoming light is not linearly polarized until just before the DIC prism. In contrast to other transmitted light interference contrasting techniques, PlasDIC does not require a compensation prism in the condenser. Finally an analyzer releases light oscillating in the same plane and thus can interfere. One slit diaphragm is sufficient to ensure λ /4 condition. The result is a clear interference image. The contrast can be adjusted, i.e. adapted to the individual specimen.
For the first time interference and polarization have been combined so skillfully that the entire specimen area is located outside the polarization-sensitive zone.
PlasDIC is the first polarization-optical differential interference technique that permits the use of plastic vessels. Standard bjectives can be used. |