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| From Simple Observation to Analysis |
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Preconfigured and individual: the systems
The demands on the relevant systems are as different as the nature of the tasks in life science research. The modular architecture of
Axio Imager 2 allows you to make an individual configuration which is exactly tuned to your requirements.
For the digital image documentation of 3 (x, y, z) to 6 dimensions (additionally t, λ and x,y location) Axio Imager 2 can be expanded with highly sensitive cameras from the AxioCam family.
AxioVision offers a large number of specific modules for subsequent image analysis.
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| AxioImager 2 with AxioVision |
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Digital intelligence: AxioVision
AxioVision is the high-performance software for useroriented solutions in digital imaging. From image acquisition and processing to image analysis and archiving. AxioVision is practice-oriented, can be operated intuitively,
and can be easily adapted to individual requirements.The modular design of the Carl Zeiss imaging software can be expanded in many ways. For example, for z-stack, multi-channel fluorescence or time-lapse images. AxioVision is the solution for growing demands.
Proven and appreciated: the AxioCam family
Carl Zeiss offers a broad spectrum of digital cameras in different performance classes. The monochrome cameras are characterized by optimum resolution and highest sensitivity (12 or 14 bit dynamics) particularly in cases of faint fluorescent samples. The color cameras stand for the best color reproduction and highest resolution up to 12 megapixels per color channel. All the cameras have thermoelectric cooling and provide the option of rapid shutter synchronization. All AxioCam cameras are characterized by rapid live image and complete integration in the Carl Zeiss system world.
Highly stress resistant: motorized focus & high-performance focus
Axio Imager 2 offers you two different versions of the z-motor. The standard design with a step size of 25 nm at a reproducibility of ± 75 nm is always part of the M-stand configuration. And for the highest requirements such as LSM or Z-stack imaging with small intervals, a highperformance focus is available for Axio Imager.Z2. It has a step size of only 10 nm with a reproducibility of ± 10 nm – and that at a 3-fold higher traverse rate. It was specifically developed for continuous use (24 hours / 7 days) and even with large stages guarantees absolutely precise focus movements over long periods.
Optical sections with ApoTome and Aurox
ApoTome has firmly established itself as the standard method in high-end research in the life sciences. For the first time it can be used with all the stands in Axio Imager 2 family: The ApoTome slider is simply inserted in the luminous field diaphragm plane of the reflected light beam path. Via the principle of fringe projection, precise optical sections are created online. With elevated contrast and clearly increased axial resolution. The ideal solution for tissue sections and thicker, fixed samples.
No stray light ever again: AxioVision 3D deconvolution
Deconvolution from Carl Zeiss calculates mathematically the stray light from outside the focal plane back to its origin. In this manner the object recorded in the 3D image stack is “unfolded”. The result is a first-class image quality particularly in samples with extremely weak fluorescence where a high light yield is essential.
Precisely on the spot: motorized stages and z-piezo insert
They allow a precisely accurate approach to positions and the highest degree of reproducibility. Via highly sensitive piezo or step motor every desired position can be exactly set and relocated.
- Piezo stage: step size 0.2 µm, reproducibility: +/- 0.6 µm
- Mechanical stage: step size 0.1 µm, reproducibility: +/- 0.3µm
- New stage control for stages with DC motors for direct coupling with the motorized stages (magnification-dependent traverse rate): highest reproducibility and precision in high-end applications
- z-Piezo focusing insert with 100 µ m focusing range for rapid imaging with Colibri and z stack images; resolution 5 nm, reproducibility: +/- 1 nm, max. additional load 2 kg, for frame size 222 x 139 mm, available mounting frames for all common preparation shapes
The scanning stages are the prerequisite for all automated
imaging techniques such as MosaiX or Mark&Find.
Precision in z: the closed loop system
Axio Imager.Z2 with the focus linear sensor offers anyone who has to fulfill extremely high requirements precision of ±1nm in the z-direction. On the one hand, the application- independent movements of the microscope stage are detected and readjusted automatically. And, on the other hand, highly precise and reproducible Z-stacks are ensured with z-steps of equal size, which gives maximum control and reliability.
New stimuli for your research: the LSM family
Confocal microscopy at the highest level: LSM 700, LSM 710 and LSM 7 MP belong to the seventh device generation of the Laser Scanning Microscope from Carl Zeiss. The use of the same first-class system components and the same software in the entire device class ensures outstanding performance and image quality without any compromises. The result: an excellent price-performance ratio. A novel beam path ensures excellent laser suppression and maximum registration of emission and results in breathtaking images. Demanding tasks such as spectral imaging, FRET, FRAP or colocalization analysis are easily managed with unprecedented image quality and high scanning speed.
LSM 710 NLO and LSM 7 MP are ideally suitable for highly sensitive deep examination of living preparations or organisms. Both systems are characterized by unrivalled sensitivity. Highly effective non-descanned detection ensures efficient depiction in deep tissue layers. These are the systems of choice for long-term developmental studies, patch-clamp- and uncaging-experiments. | |
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