| |
The Perfect Live Cell Experiment: Result Obtained Reliably and Conveniently.
| 1. Temperature Setting | 2. Heating Time |
| Set the desired incubation para- meters on the TFT display. For a perfect temperature setting, use Control Sensor T S1 directly in the culture dish. | Wait until all the incubation values have been reached. Avoid drifts simply by allowing a 2- to 3-hour warm-up period. |
 |  |
| Temperature | Heating Time |
| |
| 3. Sample | 4. Time Lapse |
| Place your sample under the microscope and select the cell that is to be observed. Avoid phototoxic effects by minimizing exposure to light, even during preparation. | Now start your time lapse experi- ment. Avoid bright ambient light to ensure detection of weak fluo- rescence signals. For extremely exacting experiments, select Incubator XL DARK S1. |
 |  |
| Sample | Time Lapse |
| |
| 5. Manipulation | 6. Analysis |
| Perform manipulations that may be required for your application, e.g. temperature changes, micro- manipulation, FRAP, uncaging, ablation, etc. | Analyze your experiment using one of the various modes of analysis in AxioVision. Benefit from the possibility of analysis during the experiment, for example with the "Physiology" module. |
 |  |
| Manipulation | Analysis |
| |
| 7. Documentation |
| Document your experiment the perfect way by saving all incu- bation data to AxioVision, directly with the image data. |
| |
|