Unmixing for tricky dual labelling experiments
The flexible Zeiss channel setups do allow many popular dye combinations: MultiChannel Unmixing

Solvig the problem of exitation crosstalk
Unmixing for tricky dual labelling experiments
Nerve cells, labelled with Cy3 and EGFP; Single Track, simultaneous excitation with 488 and 543nm and imaged in two channels 505-530nm and 560-615nm.
 
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initial channels with crosstalk
 
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clear separation with MultiChannel Unmixing