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| Emission Fingerprinting
Method available with the  LSM 510 META for the recording, analysis and separation of emission signals in multi-fluorescence imaging; working also with widely overlapping emission spectra.
( more) | |  Know How
3 Steps to get a Confocal Image
How to enhance the Image Quality
Scanning with Mercury Lamp
LSM Abbreviations
LSM Glossary |
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| Lambda Stack
Image stack with information in x, y and lambda dimensions, combinable with z and / or time series; for determination of spectral signatures at any location of a specimen.
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| Linear Unmixing
Mathematical method for the spectral deconvolution of multiple emission signals.
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| Multitracking
Scanning mode available with the LSM 5, generates multi-fluorescence images without crosstalk of emission signals, by means of fast switching between excitation laser lines, and quasi-simultaneous detection.
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| RealROI Scan
Scanning mode in which freely definable specimen areas are excited and imaged; guarantees maximum specimen preservation thanks to exact blanking of laser lines outside the selected specimen areas.
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| ROI Bleaching
Defined photobleaching of several freely defined specimen areas, e.g. for FRAP or Uncaging experiments.
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| Spline Scan
Scanning along a freehand-defined line for recording fast (physiological) processes, e.g. along neurons. | | |
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| Spot Scan
Scanning mode in which the signal intensity at a confocal point can be tracked with extremely high temporal resolution. | | |
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| Step Scan
Fast overview scan in which intermediate lines are added by interpolation. | | |
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| Tile Scan
Produces an overview image consisting of a number of tiled partial images using the motorized XY stage; for recording larger objects with improved resolution.
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