3 Steps to get a Confocal Image
	With Zeiss LSM 5 PASCAL / LSM 510 / LSM 510 META		Know How 3 Steps to get a Confocal Image How to enhance the Image Quality Scanning with Mercury Lamp LSM Abbreviations LSM Glossary
	(LSM software running, lasers and HBO turned on)
	1. View specimen in VIS mode Focus the specimen in epi-fluorescence mode using the binocular and center the part of interest; select fluorescence filter cube according to application (e.g. FITC or Cy3) via Software (window "Microscope Control "); match the field of view: change to appropriate objective magnification (consider use of correct immersion medium).
	2. Load an LSM configuration Go to LSM mode (operate tube slider or software). Open window "Configuration control", click on "Store/Apply" and select a predefined configuration from list (Single Track / Multi Track). A click on "Apply" automatically sets up the system: laser lines, attenuation, filters (EF), beam splitters (MBS, SBS), pinhole diameter, detector settings (channels, gain, offset). Or: Click on " Reuse " button (stored image/image database window) to restore settings of a previous experiment.
	3. Scan an Image Click on "Find" button (right row in window "Scan Control") => System automatically opens image window, optimizes detector settings (matches PMT gain and offset to dynamic range of 8 or 12 bit per pixel and channel), and scans an image - ready! See operating manual for scanning a stack of slices, time series etc.
	EF : Emission filter MBS : Main Beam Splitter SBS : Secondary Beam Splitter PMT : Photo Multiplier Tube