What can you do with ConfoCor 3?

	What can you do with ConfoCor 3?

	Measure any Type of Biomolecular Interactions		FCS with ConfoCor 3 What is FCS? How does FCS work? How does ConfoCor 3 work? What can you do with ConfoCor 3?

	ConfoCor 3 is a perfect tool for the analysis of molecular interactions. Measurements require only small amounts of both interaction partners and are performed in solution within seconds, overcoming any drawbacks associated to the immobilisation of ligands, as often required to perform related studies with other methods like, e.g., biosensor-based approaches. The one requisite is the availability of interaction partners that are amenable to covalent linkage of fluorescent groups. If only one of both ligands is labelled, you can perform classical FCS measurements with ConfoCor 3 and determine diffusion times, thermodynamic binding constants and on/off rate constants for the studied interaction. If both interaction partners are tagged with distinct dyes, you can exploit the full power of ConfoCor 3 and study interactions between any two binding partners without limitations imposed by too small a molecule size.

	What Types of Interactions?

	Protein-DNA Interactions

	Nucleic Acids

	Antigen-Antibody

	Receptor/ Ligand Interactions

	The Confocal Measurement Volume can be placed even inside Living Cells

	The confocal laser scanning microscope LSM 510 is used to identify the point of measurement for the ConfoCor 3. The ConfoCor 3 determines biochemical interaction at this point.





	Beyond the in vitro analysis of molecular interactions, there is an ever-growing need for new approaches to study binding phenomena in living cells. The combination of FCS and confocal laser scanning microscopy, allows for the first time to apply FCS to the non-invasive study of molecular processes in living cells. For example, you can easily track the formation of oligomeric protein complexes involving fluorescently-tagged components of signal transduction pathways.
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