Emission Separation
One way to separate fluorescent emissions is by selected, high-quality dichroic beamsplitters with a threshold wavelength lt. Thus, the beamsplitter reflects all wavelengths shorter and transmits all wavelengths longer than the threshold. Know How

Confocal Principle
Origin of Fluorescence
Fluorescence Spectra
Emission Separation
Separation of fluorescence emissions by means of dichroic filters