Result
	Fitting Mathematical Model to Correlation Curve		How does FCS work? Measurement Calculation Evaluation Result
	Direct read out:
	Mean Number of Molecules Diffusion times Triplet time Triplet fraction Bound/free ratio
	Calculated molecular parameters:
	Concentration c [mol/l] Binding constants K [mol-1] On/off-rate constants kass [mol-1s-1]; kdiss [s-1]
	Key features for FCS
	molecular interactions at equilibrium homogenous assays single molecules sensitivity fluorescence based from low molecular weight compounds to vesicles and cells conventional and ultra small volumes (down to femto liters) measuring times in the range of seconds