Gallery
The pictures below illustrate typical ApoTome applications. In each case, the conventional fluorescence image is shown in comparison to the result of image acquisition in the ApoTome mode. Please note that the quality of some of the pictures here is less than optimum, as the data have been stored as animated gif-files, a format that has its restrictions. ApoTome

Principle
Technology
System
Inside4D
Gallery
System Requirements
 
 

Start animation (264 KB)
Specimen: Section through a mouse kidney
Objective: Plan-Apochromat 63x/1.4
3-channel fluorescence
Green: Glyco-conjugate
Red: F-actin
Blue: Cell nuclei
 
 

Start animation (333 KB)
Specimen: Neuronal tissue - supra optical nucleus of a rat
Objective: Plan-Apochromat 63x/1.4
2-channel fluorescence
Green: Neurophysin II (vasopressin-expressing neurons)
Red: VGLUT2 (presynaptic terminals)
Data: Courtesy Dr. K. Mueller,
University of Cincinnati, USA
 
 

Start animation (326 KB)
Specimen: Section through the small intestine of a mouse
Objective: Plan-Apochromat 63x/1.4
3-channel fluorescence
Green: Cell nuclei
Red: F-actin
Blue: Glycocalix
 
 

Start animation (145 KB)
Specimen: Beads of a diameter of 4µm.
3D rendering of an image stack in surface mode.
Objective: Plan-Neofluar 40x/0.75
 
 

Start animation (243 KB)
Specimen: Tissue section with FISH staining. Acquisition of an image stack in ApoTome mode with subsequent 3D reconstruction (maximum projection)
Objective: Plan-Neofluar 40x/1.3
Green: Centromer chromosome Red: HER2
Blue: Cell nucleus stained with DAPI
Data: Courtesy Dr. P. Hutzler and Dr. K. Bink,
GSF Munich
 
 

Start animation (198 KB)


Start animation (200 KB)
Specimen: Drosophila larva
Objective: Plan-Apochromat 20x/0.75
2-channel fluorescence
Green: HRP
Red: Glia cells
Data: Courtesy Dr. C. Klämbt,
University of Münster
 

Start animation (200 KB)
 
 

Start animation (200 KB)
Specimen: Drosophila nervous system
Objective: Plan-Apochromat 50x
2-channel fluorescence
Green: HRP
Red: Glia cells
Data: Courtesy Dr. C. Klämbt,
University of Münster
 
 

Start animation (126 KB)
Specimen: Embryo of Ciona intestinalis, an ascidian
Objective: Plan Apochromat 20x/0.6
F-actin stained with Alexa 488 phalloidin
Data: Courtesy Marina Kerfant and
Jean-Stéphane Joly, CNRS, Gif-sur-Yvette, France
 
 

Start animation (198 KB)
Specimen: Embryo of a zebra fish
Objective: Plan-Neofluar 10x/0.3
Green: Neuron
Red: Cell adhesion molecule NCAM
Data: Courtesy Dr. Martin Bastmeier and
Monika Marx, University of Jena
Zebra Fish
as a Model Organism in Developmental Biology
 
 

Start animation (249 KB)
Specimen: Neuronal tissue (brain)
Objective: Plan - Neofluar 63x/1.3
Green: MAP2
Red: KV 2.1 potassium channel
Data: Courtesy Dr. James S. Trimmer,
State University of New York, Stony Brook
 
 
 
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