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| Beam path of the fluorescence microsocpe |
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The illustration on the left shows the beam path in the microscope with fluorescence equipment. The light travels from the powerful additional light source (1) to the exciter filter (5) via the heat-protection filter (2), the red-attenuation filter/barrier slider (3) and the luminous-field diaphragm (4). The exciter filter is integrated into the reflector slider which also contains the beam splitter (6). The dichroic beam splitter reflects the short-wave exciting light into the specimen (8) via the objective (7). The resulting emission is gathered by the objective (7) and transmitted by the dichroic beam splitter (6) because it exhibits longer wavelengths than the excitation light. The rays then also pass through the emission filter (9), where the remaining excitation light is filtered out. Therefore, this filter is also called barrier filter. As usual, the tube lens (10) and the eyepiece (11) form the microscope image, which now only consists of fluorescence light.
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