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| Accurate: calibration using a stage micrometer |
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If you want to have more exact information on the magnification, place a stage micrometer with an accurate scale – e.g. a reticle with 1/10 mm (100 µm) increments – under the objective.
Microscope objectives deviate slightly from their specified magnification, and the stage micrometer permits correction factors to be measured for the objectives. All parameters measured with the eyepiece micrometer are then multiplied by the correction factors – which only have to be determined once – making them more precise. In general, however, the reading error is larger than the inaccuracy of the magnification, particularly in the estimation of intermediate values. | |
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