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| Reflected-light – bright field |
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The beam path for the brightfield technique again includes the aperture diaphragm for reflected light (1) and the luminous-field diaphragm (2). The aperture diaphragm (1) is imaged on the objective pupil (4) via the reflector (3), and the luminous-field diaphragm (2) is imaged on the sample surface (5). Unlike reflected-light fluorescence, the reflector (3) is equipped with a neutral beam splitter which reflects or transmits white light equally well in all wavelengths. The illuminating light hits the sample surface and is reflected or scattered. The objective gathers these rays and the tube lens (6) projects the intermediate image.
The inner structure of these spherulites is barely visible in reflected-light brightfield.
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