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| Basic adjustments in the transmitted light |
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The procedure we choose will always lead to success. What we have just learned in theory will now be put into practice – following Köhler's principles. Everything is also applicable to any other microscope model, provided it is fully equipped for Köhler illumination.
Through clicking on of the following pictures, you see the labels of the microscope parts and the ray gear.
| How to prepare the microscope for adjustment |
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- The microscope configuration is complete. If the condenser features a contrasting turret, the position is on “HF” for brightfield.
- You need a sample to perform the setting. Thin, stained sections are ideal (cross sections of a plant stalk or stained tissue section). A small piece of a 35 mm photo slide (approx. 10 x 10 mm), laid on a microscope slide, flattened with a cover slip and sealed, for example with all-purpose glue, is also very useful for the purpose.
- Use the 10x objective, or 20x, if the need arises. Swing this objective into the beam path and make sure that it is tightly screwed and that the nosepiece engages correctly – since otherwise the optical axis will not be correct. Use the focusing drive to create a distance of approx. 10 mm between stage surface and objective.
- Please also have a strip of thin white paper (approx. 3 x 10 cm) ready for use.
continue with : Adjustments | |
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