Capabilities offered by the LSM software:
- Online and offline ratio for ratiometric dyes
- Online and offline F/F0 for single-wavelength dyes
- Calibration for single-wavelength and ratiometric dyes in situ and in vitro, including background correction after titration with various curve fittings according to Grynkiewicz
- Interactive scaling of image data series
- Interactive graphic display of the data measured in ROIs
- Free adaptation of the lines, areas or volumes to be displayed to the sample dimensions and orientation
- Enhanced dynamics and sensitivity compared to conventional multi-channel image acquisition, thanks to Emission Fingerprinting
- Up to 77 frames/s (512 x 32 pixels) or 2600 lines/s with LSM 710
- Up to 1000 frames/s (512 x 50 pixels) or more than 60,000 lines/s with LSM 7 LIVE
- Piezoelectric objective focusing for XYZ or XZ stacks providing high time resolution in 3D
Example:
Part of the salivary gland of a fly whose secretory cells were loaded with the Fluo-4 calcium indicator.
Changes of calcium concentration in part of the tubular salivary gland displayed as F/F0 by means of pseudocolors, with blue and red representing low and high calcium, respectively. |  |  Overview LSM
 appropriate products:
LSM 7 LIVE
LSM 710
LSM 700
Deutsche Version |