The combination of different optical sectioning and imaging techniques offers the unique possibility to build an instrument with multiple modalities to suit all your lab members or gain more information from the same samples. The modular concept and the broad range of techniques available within the Carl Zeiss portfolio allows you to find the right solution. Additionally, many technologies are available as field upgrades for combined systems. They allow you easily to increase your instrument capabilities as needed by new experiments or as a new funding becomes available.
Superresolution structured illumination (SR-SIM) and photoactivation localization microscopy (PALM) are complementary techniques with different requirements for the sample preparation. SR-SIM offers extremely flexibility both in sample type and fluorescent label while PALM provides the highest resolution for fluorescence microscopy. A combined system consisting out of these techniques provide you superresolution to easily test their samples with this new modality and advanced users to resolve structures down the tens of nanometers. Overlay the results of both imaging techniques to get a deeper understanding of your sample.
Combine LSM 710 or LSM 780 with ELYRA equipped with SR-SIM and/or PALM for maximum flexibility in terms of resolution and three dimensional, multi-color imaging. Easily switch between modalities within the same, easy-to-use, software platform. This combined system is perfect to serve the needs of multi-user facilities with diverse samples, fluorophores and experimental needs.
Whatever your live cell imaging needs, equip your microscope with spinning disk confocal, for long term, time lapse imaging, TIRF for high contrast, membrane imaging, directFRAP for kinetic and photomanipulation studies, or any combination thereof. Generate the ideal, live cell imaging instrument.
Image your samples with wide field, zoom microscopy or confocal microscopy for high resolution, multicolor, fluorescence imaging and then image the exact same area with ultraresolution, scanning electron microscopy. Perfectly overlay the light and electron images for in-depth analysis of specifically tagged proteins and structures with highly detailed, nanomorphology, providing cutting-edge information in the context of structure and function.
Microscopy techniques vary widely in strengths and benefits. The below table offers a guide for which techniques are often most suitable for particular types of samples or applications. If you have questions, do not hesitate to contact your Carl Zeiss representative, who has experience with multiple sample types and experimental needs and can guide you in the right direction.
Ask your ZEISS contact about Combined Systems now!