The new illumination and detection design of LSM 710 brings complete freedom to your fluorescence microscopy. You work with up to ten dyes and use continuous spectral detection across the complete wavelength range. LSM 710 enables confocal microscopy for a wide variety of applications.
With the inverse Axio Observer from Carl Zeiss, LSM 710 offers you unrivalled confocal microscopy in cell and developmental biology. Upright stands such as Axio Imager or Axio Examiner offer you have all the equipment you need to record neurobiological, physiological and developmental relationships to an exceptional standard.
LSM 710 gives you flexibility, allowing you to perform confocal microscopy in exactly the way you need.
The tutorial initializes with emission light (entering the window from the right) being diffracted at the surface of a grating and subsequently entering a lens that directs all spectral components to a central photomultiplier in the ZEISS QUASAR detector. In order to operate the tutorial, use the Left Slider and Right Slider controls to translate the sliding light stops back and forth in the light path to narrow the number of wavelengths reaching the multichannel photomultiplier. The Left Prism and Right Prism sliders can be used to directed a selected band of wavelengths to the auxiliary photomultipliers on the left and right hand side of the central photomultiplier.
For details go to the ZEISS Online Campus
ZEN software allows you to optimize LSM 710 automatically for your applications, delivering optimum performance. Software tools support you to calibrate the system and check central performance parameters.
The Scan Module - Core of the LSM 710
1 PTC Laser Coupling
2 Motorized Collimators
3 TwinGate Beamsplitter
5 Scan Optics
6 Master Pinhole
7 Spectral Recycling Loop
8 Beam Path
9 QUASAR Detectors
10 External Detection Port
Whenever your experiments in cell and developmental biology demand new solutions, you upgrade to additional laser lines as you need them.
With LSM 710 you profit from multicolor imaging with the newest fluorescence proteins and without spectral crosstalk. You analyze molecules and proteins – and their interactions – using any imaging method that confocal microscopy currently supports.
The high-performance 'In Tune' laser extends the spectrum of wavelengths available to your LSM 710 for confocal microscopy. You quickly and continuously set the optimum wavelength for your samples from 488 to 640 nm: excite your fluorophores exactly at the excitation maximum and with over 1.5 mW per nm.
The wavelength range and short pulses allow you to use other dyes in for example FLIM and FRET experiments. 'In Tune' works with low noise and is stable, guaranteeing sensitive detection and optimum results even when you conduct multi-channel experiments with low light. In addition, you combine 'In Tune' with additional lasers from near UV into the red range, all within your LSM 710 system.
The PTC laser concept of LSM 710 is revolutionary in confocal microscopy: the system uses pigtailed lasers directly on the scanning head. With up to eight ports, you couple near-UV, VIS and IR lasers in many combinations.
The 34-channel QUASAR detector by Carl Zeiss allows the optimal acquisition strategy for various emission spectra, without being bound to filters and dichroic mirrors – you simply configure your experiment in the software.
The spectral signal you obtain is an excellent basis for high-resolution spectral imaging and the parallel detection of up to ten colors. If needed, you can direct any parts of the spectrum to the required detector.
When it comes to excitation, the new TwinGate main beam splitter has two dichroic filter wheels and high transmission of up to 100 combinations of laser lines – allowing for the optimum fluorescence excitation of your samples.
The spectral recycling loop of LSM 710 amplifies signals by feeding the unseparated part of the emission light through the grid a second time before adding it to the complete signal. When used with the QUASAR detector, you will reduce dark noise by two-thirds and significantly improve the sensitivity of your microscope.