With Cell Observer SD, you have it all: The image quality of Axio Observer and Axio Examiner. Spinning disk technology from Yokogawa CSU-X1. The dual camera technology of ZEN software. This symbiosis of optics, hardware and software in one system makes your confocal live cell imaging uniquely accurate: you control your Cell Observer SD precisely in the millisecond range. By streaming image data, you will acquire your images in breathtakingly short times. You also document two fluorescence channels of your sensitive samples simultaneously and get even more valuable data.
The tutorial initializes with an animated cartoon of the Yokogawa optical system in the left-hand portion of the window and images of the specimen and the scanning pattern on the CCD surface on the right-hand side. In order to operate the tutorial, use the Disk Speed slider to increase or decrease the rotation speed of the dual disks and observe how it affects the CCD window. The Z-Depth slider can be used to scan through the specimen along the axial plane. New specimens can be chosen using the Specimen pull-down menu.
Excellent Optics. Image quality without compromises.
A tube lens developed by Carl Zeiss especially for Cell Observer SD gives even better color correction and higher contrast.
LCI objectives also work with a correction ring that makes the best image quality possible under your demanding conditions. Consequently, DirectFRAP – our solution for laser manipulation – is coupled into the illumination beam path. This leaves the imaging beam path free of additional components, delivering uncompromised image quality.
Two Channels simultaneously. A true Cell Observer.
Document and quantify dynamic processes in living samples with the proven Cell Observer HS technology. Your system reads two hardware-triggered, highly sensitive cameras simultaneously to give you the benefit of the highest precision in timing. Observe dynamic cell processes with high frame rates, thanks to streaming technology.
A Universe of Options. Combine freely to Your Requirements.
Combine Cell Observer SD according to your needs with motorized scanning stages, Z-Piezo inserts, stage-top incubation and DirectFRAP. All components are seamlessly integrated and managed with millisecond accuracy. You control environmental conditions when acquiring living cells with completely software-run incubation.
Drosophila Embryo Development
Drosophila embryo, early syncytical mitotic divisions. eGFP-histone Av2D, LD LCI Plan-APOCHROMAT 25x/0.8. Courtesy of Prof F. Sprenger, University of Regensburg, Germany.
Xenopus Embryo Optical Sectioning
Xenopus laevis embryo mesoderm explant, eGFP-plasma membrane staining, extended depth of focus from Z-stack. Courtesy of D. Wedlich, University of Karlsruhe, Germany.
Laser Manipulation for the Analysis of Cell Dynamics
With DirectFRAP from Carl Zeiss you manipulate fluorescent proteins in living cells and organisms for the analysis of highly active processes. With an advanced system design, resolve highly dynamic events and physiological processes in cells. Experience the shortest manipulation times for FRAP, FLIP, photoactivation and photoconversion with simultaneous manipulation across the complete region of interest.
Benefit with DirectFRAP:
Image fast processes with a wide observation field and short acquisition times for extensive data for modeling of underlying processes
Use short manipulation times with simultaneous manipulation across the complete region of interest
Combine imaging techniques, such as TIRF or spinning disk confocal, for even more extensive analysis
Not all products are available in every country. Use of products for medical diagnostic, therapeutic or treatment purposes may be limited by local regulations. Contact your local ZEISS provider for more information.