Fast and Sensitive Confocal Imaging
With Cell Observer SD, you have it all: The image quality of Axio Observer and Axio Examiner. Spinning disk technology from Yokogawa CSU-X1. The dual camera technology of ZEN software. This symbiosis of optics, hardware and software in one system makes your confocal live cell imaging uniquely accurate: you control your Cell Observer SD precisely in the millisecond range. By streaming image data, you will acquire your images in breathtakingly short times. You also document two fluorescence channels of your sensitive samples simultaneously and get even more valuable data.
The tutorial initializes with an animated cartoon of the Yokogawa optical system in the left-hand portion of the window and images of the specimen and the scanning pattern on the CCD surface on the right-hand side. In order to operate the tutorial, use the Disk Speed slider to increase or decrease the rotation speed of the dual disks and observe how it affects the CCD window. The Z-Depth slider can be used to scan through the specimen along the axial plane. New specimens can be chosen using the Specimen pull-down menu.
For more details, go to the ZEISS Online Campus
Drosophila embryo, early syncytical mitotic divisions. eGFP-histone Av2D, LD LCI Plan-APOCHROMAT 25x/0.8. Courtesy of Prof F. Sprenger, University of Regensburg, Germany.
Xenopus laevis embryo mesoderm explant, eGFP-plasma membrane staining, extended depth of focus from Z-stack. Courtesy of D. Wedlich, University of Karlsruhe, Germany.
With DirectFRAP from Carl Zeiss you manipulate fluorescent proteins in living cells and organisms for the analysis of highly active processes. With an advanced system design, resolve highly dynamic events and physiological processes in cells. Experience the shortest manipulation times for FRAP, FLIP, photoactivation and photoconversion with simultaneous manipulation across the complete region of interest.
Benefit with DirectFRAP: