Focal Charge Compensation

New Focal Charge Compensation mode for ZEISS field emission scanning electron microscopes improves image quality

High resolution 3D block face imaging for biological samples with fast acquisition rates and minimal sample damage

JENA/Germany; SAN DIEGO/USA, 2017-11-13.

In collaboration with the National Center for Microscopy and Imaging Research (NCMIR) at the University of California San Diego, ZEISS releases a new Focal Charge Compensation module for block face imaging with ZEISS GeminiSEM and 3View® from Gatan, Inc. Focal Charge Compensation expands versatility and considerably increases data quality without prolonging acquisition times and enables easy imaging of even the most charge-prone samples. Resin-embedded tissues and cells can be imaged without charging artifacts, while the pixel dwell time is reduced. Decreasing beam exposure time not only ensures fast acquisition rates, but also guards against sample damage, which is key to acquiring reliable and reproducible 3D data. Professor Mark H. Ellisman, Director of NCMIR, says “Focal Charge Compensation will breathe new life into block face scanning electron microscopy by allowing high quality imaging of previously intractable specimens, including legacy samples prepared with traditional electron microscopy stains.”


Preventing charging effects


This extension of the 3View® system from Gatan, Inc eliminates specimen charging. A gas injection system consisting of a tiny capillary needle is precisely located above the sample. Nitrogen is guided through this needle directly onto the block face surface while the chamber is maintained under high vacuum. This eliminates charging without degrading image quality. The needle retracts automatically during the cutting cycle so the workflow is uninterrupted and high acquisition rates are maintained.


Producing thousands of serial images in a single day


The 3View® system consists of an ultramicrotome directly integrated into the vacuum chamber of the ZEISS Sigma and ZEISS GeminiSEM field emission scanning electron microscopes. It enables automated serial block face imaging of embedded samples (e.g. cells or tissue) with a slice thickness down to 15 nanometers. The sample is continuously cut and imaged, and a three-dimensional rendering of the sample with nanometer-scale resolution can be reconstructed.


Charging effects are clearly visible under high vacuum, in contrast to images taken with Focal Charge Compensation, which show no charging effects even in large expanses of bare resin. The images show a ~300 micron diameter axon bundle at different magnifications. Images courtesy NCMIR.

Image Download



Press contact

Vybhav Sinha
Carl Zeiss Microscopy GmbH
Phone: +49 3641 64 3949
vybhav .sinha @zeiss .com





About ZEISS

ZEISS is an internationally leading technology enterprise operating in the fields of optics and optoelectronics. The ZEISS Group develops, produces and distributes measuring technology, microscopes, medical technology, eyeglass lenses, camera and cinema lenses, binoculars and semiconductor manufacturing equipment. With its solutions, the company constantly advances the world of optics and helps shape technological progress. ZEISS is divided up into the four segments Research & Quality Technology, Medical Technology, Vision Care/Consumer Products and Semiconductor Manufacturing Technology. The ZEISS Group is represented in more than 40 countries and has over 50 sales and service locations, more than 30 manufacturing sites and about 25 research and development centers around the globe.

In fiscal year 2015/16 the company generated revenue approximating €4.9 billion with over 25,000 employees. Founded in 1846 in Jena, the company is headquartered in Oberkochen, Germany. Carl Zeiss AG is the strategic management holding company that manages the ZEISS Group. The company is wholly owned by the Carl Zeiss Stiftung (Carl Zeiss Foundation).


Further information at www.zeiss.com







Microscopy

The Microscopy business group is the world's only one-stop manufacturer of light, electron, X-ray and ion microscope systems. The portfolio compromises solutions and services for the life sciences and materials research as well as for industry, education and clinical practice. The business group is headquartered in Jena. Additional production and development sites are located in Oberkochen, Göttingen and Munich, as well as in Cambridge in the UK, and in Peabody, MA and Pleasanton, CA in the USA. The business group is allocated to the Research & Quality Technology segment. Around 6,100 employees work for the segment, generating revenue totaling €1.5 billion in fiscal year 2015/16.






We use cookies on this site. Cookies are small text files that are stored on your computer by websites. Cookies are widely used and help to optimize the pages that you view. By using this site, you agree to their use. more