Super-resolution microscopy by single-molecule photoactivation or photoswitching and position determination (localization microscopy) has the potential to fundamentally revolutionize our understanding of how cellular function is encoded at the molecular level. Among all powerful high-resolution imaging techniques introduced in recent years, localization microscopy excels at it delivers single-molecule information about the distribution and, adequate controls presupposed, even absolute numbers of proteins present in subcellular compartments. This provides insights into biological systems at a level we are used to think about and model biological interactions. We briefly introduce basic requirements of localization microscopy, its potential use for quantitative molecular imaging, and discuss present obstacles and ways to bypass them. I will demonstrate the advantageous use of single-molecule localization microscopy by dSTORM for quantitative imaging of plasma membrane receptors and the molecular architecture of multiprotein complexes including imaging by 3D lattice-light-sheet dSTORM. In addition, I will show how single-molecule localization microscopy can be used advantageously to improve next generation immunotherapies. Finally, I will show how dSTORM in combination with expansion microscopy can pave the way for super-resolution imaging with true molecular resolution.

Universität Würzburg​, Campus am Hubland​, am Naturwissenschaftlichen Hörsaalgebäude ​, Theodor-Boveri-Weg 1​, 97074 Würzburg​

Keeping the data connected and accessible during a complex imaging workflow becomes increasingly important, even more so if several users are involved. ZEN provides integrated solutions for data management and data fusion which help microscope users in their daily workflows. ​
The ZEN Connect module allows a seamless fusion of data from different instruments not only in 2D but also in 3D, while keeping a track of the data in well-structured projects. This is complemented by ZEN Data Storage which allows a central server-based access and sharing of images. ​
To extract information from images the ZEN ecosystem platform provided various powerful tools to segment and analyze images. Using ZEN Intellesis together with www.apeer.com platform enables users to train and deploy their own machine-learning models and integrate them into different workflows incl. tools like ZEN Guided Acquisition, where the Image Analysis is used to guide the microscope automatically to your regions of interest.

Universität Würzburg​, Campus am Hubland​, am Naturwissenschaftlichen Hörsaalgebäude ​, Theodor-Boveri-Weg 1​, 97074 Würzburg​

ZEISS is offering individual hands-on sessions to test new functionalities in microscopy and talk to experts about your needs. Within a one-hour demonstration, you can personally gain insight into connected microscopy from ZEISS.

ZEISS Elyra 7 with Lattice SIM²
Live Imaging with Unprecedented Resolution
The super-resolution microscope Elyra 7 takes you far beyond the diffraction limit of conventional microscopy: With Lattice SIM² you can now double the conventional SIM resolution and discriminate the finest sub-organelle structures, even those no more than 60 nm apart. You don‘t need to sacrifice resolution when imaging at high speed using only the minimal exposure needed for life observation. Elyra 7 enables you to combine super-resolution and high-dynamic imaging – without the need for special sample preparation or expert knowledge of complex microscopy techniques.

Universität Würzburg​, Campus am Hubland​, am Naturwissenschaftlichen Hörsaalgebäude ​, Theodor-Boveri-Weg 1​, 97074 Würzburg​

Universität Würzburg, Biozentrum Hubland, Lehrstuhl Biotechnologie/Biophysik Prof. M. Sauer, room C 323