ZEISS Introduces Lightsheet 7

Your light sheet microscope for Multiview imaging of living & cleared specimens

Lightsheet 7 is your light sheet fluorescence microscope for fast and gentle imaging of whole living model organisms, tissues, and developing cells. The exceptional stability of Lightsheet 7 lets you observe living samples over extended periods of time – even days – with less phototoxicity than ever before. And now you can employ this technique to image large optically cleared specimens, e.g. whole mouse brains, with subcellular resolution.

Lightsheet 7 is your boxed, stable turnkey system for light sheet fluorescence microscopy (LSFM), providing you with:
 

ZEISS Lightsheet 7
  • Increased Flexibility: image specimens at up to 2 cm in size at any refractive index between 1.33 - 1.58, and in almost all clearing solutions.
  • Enhanced Image Quality: newly designed optics & sample chambers let you adjust to the perfect refractive index. Software tools ensure the right adjustment of imaging parameters, zoom settings, tiles, positions, and data processing parameters. The patented Pivot Scan technology delivers artifact-free optical sections.
  • Increased Sensitivity: new pco.edge sCMOS detectors enable observations of the fastest processes at lowest illumination light levels. Get a real life view of your samples without adverse effects of excitation light on their biology.
ZEISS Lightsheet 7
ZEISS Lightsheet Technology

Increased Flexibility

ZEISS Lightsheet Technology

Which optical clearing method you choose will depend on the type of tissue you are imaging, your fluorescent labels and the size of the sample itself. Lightsheet 7 is designed to match all of these different conditions. You can now image specimens at up to 2 cm in size at any refractive index between 1.33 and 1.58, and in almost all clearing solutions. Lightsheet 7 lets you acquire overview images and data with subcellular resolution.

Image optically cleared large tissue samples, organoids, spheroids, organs, brains, developing organisms, and other specimens.

Enhanced Image Quality

Lightsheet 7 Plan Overview

Take your LSFM imaging a step further to tackle a broad range of applications and achieve best image quality. Newly designed optics and sample chambers let you adjust to the perfect refractive index. The new sample holder makes mounting larger specimens simple. Smart software tools help you adjust imaging parameters, such as light sheet and sample positions, the right zoom settings, tiles and positions as well as data processing parameters.

All of these new features go hand in hand with the reliable ZEISS combination of cylindrical lens optics and laser scanning to generate the illumination light sheet. Add the patented Pivot Scan technology and get artifact-free optical sections with best image quality.

Lightsheet 7 Plan Overview
Development of Arabidopsis
Development of Arabidopsis. Courtesy of S. Valuchova, P. Mikulkova, and K. Riha, CEITEC, Masaryk University, Brno, Czech Republic.

Increased Sensitivity

Development of Arabidopsis
Development of Arabidopsis. Courtesy of S. Valuchova, P. Mikulkova, and K. Riha, CEITEC, Masaryk University, Brno, Czech Republic.

Lightsheet 7 now features the high quantum efficiency of pco.edge sCMOS detectors to enable observations of the fastest processes at the lowest illumination light levels. You will get a real life view of your samples without the adverse effects of excitation light on their biology. For vertically oriented specimens and highest frame rates, opt for the CMOS detector Axiocam 702: a special sample chamber provides heating, cooling, and CO2 to maintain the perfect environment for your experiments.

Add Multiview and triggering options to control external devices – Lightsheet 7 is your ideal light sheet microscope to observe live processes in an almost unlimited range of organisms.


Dive into the Details

Explore the features & applications of Lightsheet 7 in more detail

ZEISS Lightsheet 7 Product Brochure

Product Brochure

ZEISS Lightsheet 7

Join the Webinar

BiteSize Bio Webinar

Light Sheet Microscopy with Lightsheet 7

ZEISS Lightsheet 7 - Immersion Media & Clearing Agents White Paper

Technology Note

How to Get Best Image Quality with Various Types of Immersion Media & Clearing Agents

 

Animation Video

Principle of ZEISS Lightsheet 7

Get in Contact with Us

Speak to our light sheet microscopy experts about your questions

Get to know how ZEISS Lightsheet 7 benefits your research or facility, book a demo at our customer centers, or get a quote.
We are looking forward to hearing from you.

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