Isotropic Volume Imaging

Virtual System Demonstration

March 10, 2021 | 10 - 11 am (CET)

Long-term volumetric imaging of living cells

ZEISS Lattice Lightsheet 7

The complexity of life is the result of the dynamic interplay within and between molecules, organelles, cells and tissues. Deciphering the underlying processes and principles has always been the most prominent pacer, driving technological breakthroughs in microscopy. Our portfolio allows scientists to capture all these levels of complexity with maximum gentleness. High-speed volume acquisition with minimal light exposure and almost isotropic resolution is achieved by novel ZEISS Lattice Lightsheet 7.

In this workshop we will summarize the technical background of lattice lightsheet imaging and applications in fluorescence microscopy. A introductory presentation will be followed by a hands-on session and discussion.

Pollen study 4th trial Mitotracker Green LysoTracker Red Image © Sample courtesy of R. Whan, UNSW, Sydney, Australia

Key Learnings

  • Gentle long-term imaging of living specimen
  • High-speed volumetric imaging
  • Nearly isotropic resolution for high quality 3D visualization & quantification

Program

  • ZEISS Lattice Lightsheet 7 - Dr. Hubert Bauch (15 min Presentation)
  • System Demonstration - Dr. Steffen Burgold (30 min)
  • Q&A (15 min)

Speakers

Hubert Bauch

Hubert Bauch studied biology with a focus on cell biology in Bonn, Germany. He received his PhD in neuronal developmental biology at the Natural and Medical Sciences Institute (NMI) at the University of Tübingen in Reutlingen, Germany. Since 1998, he has been working at ZEISS Microscopy as a specialist for various tasks and applications in the field of software and imaging.

Dr. Hubert Bauch ZEISS Research Microscopy
Steffen Burgold

Steffen Burgold studied biotechnology at the University of Applied Sciences Jena, Germany and worked as a process engineer at the startup company Novosom AG in Halle, Germany. In his postgradual master studies in photonics he emphasized on light microscopy techniques and did his thesis at the Charité, Berlin with Prof. Michael Schaefer. He moved on with his PhD and postdoc in neurobiology (Alzheimer disease) with Prof. Herms at the University of Munich and the German Center for Neurodegenerative Diseases, Germany. Here, Steffen established a correlative workflow from intravital microcopy to FIB-SEM imaging of the brain ultrastructure. Since 2017 Steffen has been working as application specialist in the Zeiss Microscopy Customer Center Europe.

Dr. Steffen Burgold ZEISS Research Microscopy

Watch the Webinar Recording