Isotropic Volume Imaging

Virtual Workshop

January 26 - 27, 2021

Unravel Life, without Compromises – Solutions for Life Sciences Research

The New Lattice Lightsheet 7

Convince yourself how your work can benefit from volumetric imaging of subcellular structures and dynamics over hours and even days with next to no photo damage. Dr. Roland Thünauer, University of Hamburg, Germany, will discuss the scientific challenges it can tackle and its advantages for research, based on his own experience with a ZEISS Lattice Lighsheet system from our First User Program. 

Other talks will cover the wide range of applications of Light and Electron Microscopy in Life Cell Imaging as well as our flexible software solutions.  
Discuss all your questions with our application specialists and product managers regarding the ZEISS Lattice Lightsheet 7 and other state-of-the-art technologies.

 

Pollen study 4th trial Mitotracker Green LysoTracker Red Image © Sample courtesy of R. Whan, UNSW, Sydney, Australia

Agenda

Day 1 - January 26, 2021
Time (CET) Topic Speaker/Operator

1:30 – 2:00 pm

Unraveling Nature's Dynamics with ZEISS Gentle Live Cell Imaging Solutions

Dr. Frank Vogler,
Application Specialist Automated and Confocal Imaging

2:00 – 2:45 pm

Infection Biology Applications of Lattice Light Sheet Technology

Dr. Roland Thünauer,
Head of ALFM Facility at the Centre for Structural Systems Biology in Hamburg
 

Day 2 - January 27, 2021
 
Time (CET) Topic Speaker/Operator

1:30 – 2:00 pm

Correlate Functional and Ultrastructural Information in a Multidimensional Context

Dr. Eric Hummel,
Global Marketing Business Sector Life Sciences

2:00 – 2:45 pm

ZEISS Software: From Acquisition to Maintenance

Dr. Uros Krzic,
Product Manager for Software Solutions

4:45 – 5:00 pm

Wrap-up & Good-bye

 

DAY 1 | January 26, 2021

2 Talks

January, 26 2021
1:30 p.m. – 2:45 p.m.
Virtual Workshop

First results of infection biology applications

Dr. Roland Thünauer

The Advanced Light and Fluorescence Microscopy (ALFM) Facility at CSSB Hamburg operates a ZEISS Lattice Lightsheet 7 microscope since October 2020. The microscope is located in a biosafety level 2 laboratory. In this presentation we will report on a range of first infection biology applications of lattice lightsheet microscopy spanning from monitoring the intracellular fate of viruses to imaging Plasmodium parasite development in red blood cells.

Date and Time

January 26, 2021
2:00 p.m. – 2:45 p.m.

Dr. Roland Thünauer Head of ALFM Facility at the Centre for Structural Systems Biology in Hamburg

Unraveling Nature's Dynamics

Dr. Frank Vogler

The complexity of life is the result of the dynamic interplay within and between molecules, organelles, cells and tissues. Deciphering the underlying processes and principles has always been the most prominent pacer, driving technological breakthroughs in Microscopy. Our portfolio allows scientists to capture all these levels of complexity with maximum gentleness. High-speed volume acquisition with minimal light exposure and almost isotropic resolution is achieved by our novel ZEISS Lattice Lightsheet 7. Breaking the boundaries of optical resolution with up to 255 frames per second resolves fastest cellular dynamics and is achievable with ZEISS Elyra 7. The ZEISS Lightsheet 7 unravels the complexity in living organisms and provides insights into the body architecture for a whole range of cleared specimen. Our fully automated throughput platform, the ZEISS Celldiscoverer 7, facilitates to study quantitative dependencies in up to 1536-well formats. In this workshop we will show how the latest ZEISS live imaging portfolio will leverage your imaging experiments now and in the future.

Date and Time

January 26, 2021
1:30 p.m. – 2:00 p.m.

Dr. Frank Vogler Application specialist for automated and confocal microscopy.

DAY 2 - January 27, 2021

2 Talks

January 27, 2021
1:30 p.m. – 2:45 p.m.
Virtual Workshop

Correlate Functional and Ultrastructural Information in a Multidimensional Context

Dr. Eric Hummel

Life science research for electron microscopy increasingly demands 3D ultrastructural imaging methods to obtain comprehensive data about cellular ultrastructure. Focused ion beam, 3D array tomography and serial blockface imaging scanning electron microscopy are arising techniques for three dimensional visualization of biological structures. All these methods enlighten the understanding of cells on ultrastructural level in 3D dimensions. A prospect in modern microscopy is the potential to augment fluorescent imaging with direct high resolution ultrastructural information provided by electron microscopy. Such correlative light- and electron microscopic techniques rely on different localization strategies. ZEISS also enter new ways in correlative and 3D ultrastructural imaging with close to native cryo imaging methods. The development of 3D correlative workflows combine both methods in a perfect way and enable scientists to correlate functional and ultrastructural information in a multidimensional context.

Date and Time

January 27, 2021
1:30 p.m. – 2:15 p.m.

Dr. Eric Hummel Global Marketing Business Sector Life Sciences

From Acquisition to Maintenance

Dr. Uros Krzic

Software plays an essential role in our daily life, and also in Microscopy, its influence is growing exponentially. At ZEISS, we continually develop software to aid the microscope users. With ZEN as the basis for all our systems, we offer flexibility and computing ower to handle even the most complex systems. But several modules are available to support researchers after the image acquisition. ZEN Connect allows for data fusion to provide more extensive insights into your samples by combining data from different imaging modalities. With APEER, scientists can easily create workflows to process & analyse data employing cloud computing. Furthermore, it allows for the implementation of machine learning protocols in microscopy, something we also perform with the ZEN Intellesis Module. These topics, and more, will be discussed during this talk.

Date and Time

January 27, 2021
2:15 p.m. – 2:45 p.m.

Dr. Uros Krzic Product Manager Software Solutions