A New Confocal Experience

Watch the on-demand version of the event stream here

Share this event video with your friends & colleagues:

Discover LSM Plus and Airyscan jDCV and their technology in more detail here:


Laser scanning microscopy is valued for its high-quality generation of optical sections and has long set the imaging standard for a wide variety of samples and experiments.

What if you could improve the data quality of your confocal microscope while fully preserving its ease of use and flexibility?

Be introduced to a new confocal experience, improving your confocal imaging with ease across all available detection modes and emission spectra. See how:

  • improved confocal imaging allows you to push your experiments and be more productive
  • an improved resolution of Airyscan enables identification of new structures in your samples
  • first users have already benefitted from these new capabilities to further their experimental goals

Hosts and Guests

Rosy Manser

Rosy Manser

Host
Sector Life Sciences
ZEISS Microscopy

Annette Bergter

Annette Bergter

Host
Sector Life Sciences
ZEISS Microscopy

Guests

Imperial College London

Prof. Cristina Lo Celso

Professor of Stem Cell Biology

Faculty of Natural Sciences, Department of Life Sciences

Co-Director

Faculty of Medicine, Centre for Haematology

Group Leader

Sir Francis Crick Institute

Mr. George Adams

PhD Student

Faculty of Natural Sciences, Department of Life Sciences

Ms. Marine Secchi

PhD Student

University of California, Davis

Dr. Kelly Subramanian

Post Doctoral Scholar (Professor Jodi Nunnari Lab)

Department of Molecular and Cellular Biology

Mr. Joseph Williams

PhD Student (Professor Scott Dawson Lab)

Department of Microbiology and Molecular Genetics

Westfälische Wilhelms-Universität Münster

Dr. Thomas Zobel

Imaging Network

Cells in Motion Interfaculty Centre

Your Questions during the Stream

During the launch event you asked many interesting questions – thank you for your great interest in LSM Plus and Airyscan Joint Deconvolution.
Please find answers to your questions here. If you have additional questions, please get in contact with us.

Is LSM Plus using Wiener deconvolution?

Yes, we use a Wiener filter DCV for LSM Plus.
 

Is LSM Plus a post-processing algorithm?

LSM Plus is fully integrated into the LSM workflow. Sampling is optimized for acquisition. The second step is post-processing with the LSM Plus Wiener filter that provides an easy-to-use online preview function.

Is LSM Plus based on a neural network Ai Algorithm?

LSM Plus is a linear Wiener filter deconvolution which does not need any training. The needed optical information of the system and beam path settings are provided within our algorithm and are considered automatically. So, no AI algorithm is needed.
 

What exactly does this one button do? Is it a deconvolution method? How do you look at the actual processing?

The LSM Plus button sets the optimal sampling for the acquisition. The LSM Plus viewer performs the processing, either choosing a good setting automatically, or with manual control of one slider. A new image can be directly created and viewed in an image container.
 

Is it possible to show the PSF before and after LSM Plus?

There are no special tools for this in LSM Plus, but the ZEN software can manually display a PSF of beads before and after any image processing. The Ortho View or the special PSF wizard in the ZEN Deconvolution module can be used.
 

Is there any speed sacrifice with LSM Plus?

No, you can use all imaging speeds available.
 

What is the increase in resolution?

LSM Plus resolves 160 nm (pinhole at 0.8 AU) and 120 nm (pinhole at 0.3 AU). These values were measured with nanorulers (GATTA-SIM 160B, GATTA-SIM 120B).
 

Is this an application of the SIM² algorithm to regular confocal?

LSM Plus is different from SIM² and works only with confocal images. The only thing in common is that both methods are based on deconvolution. For more details, please refer to the white paper “A Practical Guide of Deconvolution”.
 

Were the NIR images taken using the detectors and optics on an LSM 900 or 980 or another microscope?

The NIR data were acquired with LSM 980, using the NIR detector (up to 900 nm emission range) and the 700 nm laser line.
 

Can you then carry quantification on the processed images?

LSM Plus (Wiener filter DCV) and Airyscan Joint Deconvolution provide quantitative results – so you can perform quantification measurements after processing. For more details, please refer to the white paper “A Practical Guide of Deconvolution”.
 

Is LSM Plus also available for Airyscanning / Multiplexing?

LSM Plus is for confocal images and is based on the proven Wiener filter that we use as well for Airyscan SR and Multiplex processing. Airyscan needs an additional pixel reassignment process for the 32 elements, called Sheppard Sum. Airyscan data is therefore optimally processed. For more details, please refer to the white paper “A Practical Guide of Deconvolution”.
 

What is the field of view?

The FOV for LSM Plus and Airyscan Joint Deconvolution is not changing compared to confocal and Airyscan SR imaging.
 

Will it work on the LSM 880?

LSM Plus processing is not working with data acquired with LSM 880. Airyscan SR data acquired with LSM 880 can be processed with Airyscan jDCV. See compatibility table.
 

Can LSM Plus be added on to an older generation LSM?

LSM 800, 900 and 980 can be upgraded with LSM Plus. And you can process LSM data you already acquired with ZEN (blue edition). See compatibility table.
 

Is LSM Plus option for ZEN 3.5 only?

Yes, LSM Plus requires ZEN release 3.5.
 

So, Airyscan is superresolution?

Yes, Airyscan Superresolution (SR) mode achieves 120 nm. Airyscan Joint Deconvolution (jDCV) reaches 90 nm lateral resolution. These values were measured with nanorulers (GATTA-SIM 120B, GATTA-SIM 90B).
 

What is the Joint Deconvolution? Richardson-Lucy + Wiener?

Airyscan jDCV is based on Richardson-Lucy DCV. Wiener filter is the DCV used for Airyscan SR processing. They are not combined. For more details, please refer to the white paper “A Practical Guide of Deconvolution”.
 

Is Airyscan used for acquisition or is it for post-processing Like LSM Plus?

For Airyscan jDCV, the Airyscan detector is used for acquisition in super-resolution mode.
 

Does Joint Deconvolution work with old Airyscan images?

Yes, old Airyscan SR data can be used for Joint Deconvolution, independent of ZEN (black or blue edition).
 

Can I add that function to our LSM 980?

Yes, LSM Plus and Airyscan jDCV can be added to existing LSM 980 systems. Please contact your local ZEISS specialist for more information. See compatibility table.
 

Please provide the white paper for joint deconvolution.

You find the white paper covering all ZEISS deconvolution methods, including Airyscan jDCV and LSM Plus, here: “A Practical Guide of Deconvolution”.
 

For more information on LSM Plus and Airyscan Joint Deconvolution, visit the following webpage.

Get in Contact with Us

Do you have questions or want to upgrade? Get in contact with one of our confocal imaging specialists. We look forward to hearing from you!

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