ZEISS Microscopy

4D Investigation of Microdamage Initiation & Progression in Cartilage-Bone Interfaces

With X-ray Tomography

4D Investigation of Microdamage Initiation & Progression in Cartilage-Bone Interfaces

With X-ray Tomography

Analyzing mechanical performance & interplay

Of biological tissues

The Use Case: Osteoarthritis (OA) is one of the most prevalent and disabling degenerative diseases affecting millions of people worldwide. Despite years of research, understanding the etiology and pathogenesis of OA remains unclear. It has been speculated that the OA process may be triggered by altered strain transfer in the osteochondral unit. To investigate this further, a deep
understanding of the cartilage-bone mechanics is of vital importance, especially their interface.

The Challenge: Investigation of the cartilage-bone interface with X-ray computed tomography (XCT) is challenging due to the different absorption of those two tissues. As an alternative to the well-established imaging with absorption contrast, propagation-based phase-contrast X-ray imaging has emerged as a technology capable of resolving unstained soft tissues and biomaterials.

The Solution: In the white paper, ZEISS Xradia Versa enabled high-resolution X-ray Computed Tomography (XCT), in situ mechanical testing and digital volume correlation (DVC), providing a comprehensive toolset for analysis of the mechanical performance of biological tissues and biomaterials.

The Use Case: Osteoarthritis (OA) is one of the most prevalent and disabling degenerative diseases affecting millions of people worldwide. Despite years of research, understanding the etiology and pathogenesis of OA remains unclear. It has been speculated that the OA process may be triggered by altered strain transfer in the osteochondral unit. To investigate this further, a deep understanding of the cartilage-bone mechanics is of vital importance, especially their interface.

The Challenge: Investigation of the cartilage-bone interface with X-ray computed tomography (XCT) is challenging due to the different absorption of those two tissues. As an alternative to the well-established imaging with absorption contrast, propagation-based phase-contrast X-ray imaging has emerged as a technology capable of resolving unstained soft tissues and biomaterials.

The Solution: In the white paper, ZEISS Xradia Versa enabled high-resolution X-ray Computed Tomography (XCT), in situ mechanical testing and digital volume correlation (DVC), providing a comprehensive toolset for analysis of the mechanical performance of biological tissues and biomaterials.

Full-field residual strain distribution computed after mechanical testing. Equivalent von Mises Strain (εeq) distribution for a) a cross-section of the sample, b) the entire volume, c) the articular cartilage and d) mineralized tissue computed using DVC. Figure courtesy of: Tozzi et al.. Materials 2020;13(11).

Download the White Paper

4D Investigation of Cartilage-Bone Interface

About the white paper authors & their institutions

Driving forward biooengineering for bio-inspired materials

Gianluca Tozzi | Zeiss Global Centre, School of Mechanical and Design Engineering, University of Portsmouth, UK

The school is driven to make the world a better place through smarter thinking and design. One area of expertise is biomedical engineering, where the school is working at the interface of engineering, life sciences and biomedical sciences to deliver research with socioeconomic impact – including health technology and bio-inspired materials.