The Use Case: Regeneration of damaged tissue via nanofibrous scaffolds requires designing an extracellular matrix-like scaffold with high surface area to volume ratio as well as high porosity for promoting homogeneous cell attachment and proliferation throughout the scaffold. Characterizing these sample features is indispensable for the design and fabrication of tailored scaffold materials.
The Challenge: 3D characterization of the porous structure of the cross-linked gelatin scaffolds using X-ray microscopy is a challenge, because the gelatin nanofibers are composed of elements with low atomic number that deliver very low X-ray absorption-contrast.
The Solution: In the white paper, ZEISS Xradia 810 Ultra was successfully applied to characterize the morphology of electrospun gelatin fibers for understanding the effect of fiber cross-linking in the gelatin mat morphology.