Seeing is Believing 2015

Microscopy Events

ZEISS at Seeing is Believing 2015

October 6 - 10, Heidelberg, Germany

Visit us and discover new insights to processes of life.
Learn how ZEISS light, electron/ion and X-ray microscopes give you the power to work on any sample.

Join us in workshop & discussion sessions and learn from our microscopy experts about the newest advances in confocal microscopes.

 

Learn in workshop session 2 about newest ZEISS technologies & applications

  • Request a Demo
    LSM 800 with Airyscan
    • Perfectly tailored to your needs
    • Open interfaces to extend your system
    • Your compact system for high-end confocal imaging

     

    Book a hands-on demonstration here!

  • Talk & Discussion
    Tuesday | October 6th  - 2015 | 12:15 - 13:15
    ZEISS Workshop on Detection Concepts in Confocal Imaging - Session 2
    Warm-up Talk
    Modern detectors in confocal microscopy and their applications
    U. Krzic room number will be announced soon
    Open Discussion
    Optical vs digital imaging or how to have the best of both worlds – current status and trends in confocal detection concepts
    B. Zimmermann
    R. Wolleschensky
    R. Ankerhold
    room number will be announced soon
  • Abstract

    ZEISS Workshop on detection concepts in confocal microscopy
    Dr. Uros Krzic, Dr. Richard Ankerhold / Carl Zeiss Microscopy GmbH

    Since confocal scanning microscopy was invented it has developed into an indispensable and widely spread tool for microscopic imaging in biomedical research. While confocal microscopy is nowadays a matured technology available off-the-shelf from several microscope producers, it is still subject to rapid development. Almost every year we witness new improvements that push the limits in terms of speed, sensitivity, resolution, functionality and versatility.
    The development has been in recent years largely driven by advances in detector technologies. Most notably, introduction of gallium arsenide phosphide (GaAsP) photocathode led to a new generation of detectors with an unmatched sensitivity. The combination of PMT (photomultiplier tube) and APD (avalanche photodiode) technologies gave rise to a photodetector (hybrid photodetector), that is also well suited for time-resolved measurements. The availability of multi-detector PMT arrays opened a myriad of new possibilities in hyper-dimensional imaging. The latest development is the Airyscan detector. This novel detection technique exploits the design of a sensitive GaAsP detector array to scan over the Airy disk (hence the name). It replaces the pinhole as the core component of a conventional confocal microscope by imaging the fluorescent spot simultaneously onto a multitude of detectors. Thus, the intrinsic drawback of classical confocal imaging sacrificing fluorescence light for sectioning and resolution could be eliminated. The reassignment of the detected fluorescence at each detector subunit produces images with boosted signal-to-noise and a resolution improvement. In other words, an experimenter is able to see more detail with less light, using the same sample preparation procedures, dyes and acquisition conditions. Airyscan is a very flexible and powerful detector concept.
    In the first part of the workshop we will present an overview of currently used confocal detectors and detection concepts, and their strengths and weaknesses in biomedical imaging. The second part of the workshop will be dedicated to an open discussion on future directions in confocal detection concepts, with special focus on synergies between advanced detectors and signal processing. There has been a high innovation rate in software and processing algorithms in microscopy that can be applied for instance in conjunction with particular illumination and detection schemes. What is the future potential of these approaches in biomedical imaging? How can we benefit from the best of both worlds – modern detector hardware blended with clever digital processing?

  • Venue

    European Molecular Biology Laboratory (EMBL)
    Advanced Training Center
    Meyerhofstraße 1
    69117 Heidelberg
    Germany

Revolutionize Your Confocal Imaging

LSM 8 Family

Revolutionize Your Confocal Imaging

The LSM 8 family with optional GaAsP detection and Airyscan delivers supreme image quality with highest sensitivity for quantitative imaging in life sciences.
The new confocal laser scanning microscopes offer high sensitivity, enhanced resolution in x, y and z, and high image-acquisition speed in one system.

Airyscan

Conventional Confocal vs Airyscan

ZEISS Airyscan utilizes a new detection concept that uses an array detector to oversample each Airy disk in order to gain sensitivity, resolution and speed for higher throughput. 
Fixed tumor cells, tubulin labelled with Alexa 555, comparison of conventional confocal vs Airyscan SR microscopy with ZEISS LSM 880. Sample courtesy of P. O`Toole and P. Pryor, University of York, UK.


If you have further questions, please contact:
E-mail: 
 workshops .microscopy .de @zeiss .com