ZEISS at ELMI 2016

Microscopy Events

ZEISS at European Light Microscopy Initiative (ELMI) 2016

May 24 - 27, Debrecen, Hungary

Visit us at the ZEISS room (#105) at ELMI and explore customized solutions precisely tailored to your daily work.

Discover Your New Standard for Fast and Gentle Confocal Imaging - LSM 880 with Airyscan and the New Fast Module.

ZEISS Workshops at ELMI

Join ZEISS hands-on workshops and see how to enter new dimensions of enhanced optical resolution in combination with full sample flexibility or how to boost Speed and Sensitivity with Lightsheet microscopy.
Don´t miss your chance to use real equipment and samples, while also exploring a variety of practical topics in common to day-to-day microscopy work.

Please visit your ELMI account in order to sign up for the hands-on workshops.

LSM 880 with Airyscan

Drosophila embryo. Color coded maximum intensity projection of the central nervous system. Courtesy of J. Sellin, AG Hoch, LIMES Institute, Bonn, Germany

ZEISS Workshops
Boosting Speed and Sensitivity in Light Microscopy

Workshop 1, 3 and 5
Room - 105

Speed and sensitivity are major and interrelated parameters, which empower researchers to follow even more ambitious strategies in analyzing and dissecting static structures and dynamic processes in living or fixed samples. From a microscope technology perspective, the obsession is clear: in most applications, we want higher sensitivity, better signal to noise, and faster image acquisition. One might think that speed or sensitivity have reached a level of saturation beyond which no further improvement is possible, yet smart new concepts and their intelligent implementation help us break through this imaginary wall. In Lightsheet microscopy, the speed of 3D-image acquisition reaches unmatched levels by changing from the confocal principle to oblique illumination for optical sectioning in the z-dimension. In the Lightsheet Z.1, this allows switching from scanning to wide field image acquisition with fast and high speed digital cameras, while still obtaining precise positional information in z. Due to the speed and low excitation light exposure, very large z-stacks of several thousand sections in z are no longer a problem. This also opens new routes, not only in fast imaging of living organisms, but also large samples such as cleared brains. However, confocal microscopy is not willing to concede at all. In our LSM 880 with Airyscan, new detector technologies and methods to extract information from the obtained data, enable us to improve scanning speed to surprising levels, while not giving up the benefits of low noise and high resolution, and many more tools, which are unique to scanning systems. In this workshop, we will explain and demonstrate, how optical sectioning is achieved in Lightsheet and confocal microscopy and how you can use these technologies to expand the spectrum and potential of your own research projects

Abstract | Workshop 1,3 and 5
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Lightsheet Z.1

HBE-Spheroids with GFP-tagged adenoviral infection; Courtesy of Greber Group, Vardan Andriasyan, Artur Yakimovich, University of Zürich/ZEISS Microscopy Labs

ZEISS Workshops
Entering new dimensions of enhanced optical resolution in combination with full sample flexibility
Workshop 2, 4 and 6
Room - 105

In light microscopy, optical resolution is limited by diffraction - as formalized in 1873 by Ernst Abbe, professor of physics at the University of Jena and co-founder of the Carl Zeiss company. Thus, an image of a point-like object (such as a GFP molecule) is never a point, but an infinite pattern of light: the “Airy pattern” - which is described by the point spread function (PSF). Under normal circumstances, the optical resolution of confocal microscopes is limited by the geometry of the Airy pattern. To obtain structural information beyond this limit, ZEISS has implemented with Airyscan a new and revolutionary detection technique that can be added as an option to our Laser Scanning Microscopes (LSM). In simple terms, an array of highly sensitive GaAsP detectors allows scanning over the Airy disk resulting in a dataset giving an improved resolution down to values of e.g. 140nm laterally and 400nm axially with 488nm illumination. This technique is extremely simple to use and benefits all typical LSM applications including multiphoton approaches. Thereby no specific dyes or preparation methods are required. Thus, Airyscan microscopy offers a maximum flexibility of choosing samples for microscopy beyond the classical resolution limit. In a Lightsheet microscope, resolution is not only limited by the laws of optics, but also by the structure of your sample. Photons emitted e.g. by a GFP molecule inside of a living embryo have to travel through the specimen before they even reach the microscope objective. Nevertheless, subcellular structures can be observed in living and intact samples. Furthermore, multi-view imaging can enable nearly isotropic resolution in all dimensions. Combined with sample integrity, flexibility, and minimal light exposure, the Lightsheet Z.1 therefore allows for experiments, which were unthinkable in the past. In this workshop we will discuss and demonstrate how to improve resolution in LSM 880 with Airyscan, and in Lightsheet Z.1.
 

Abstract | Workshop 2, 4, and 7
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If you have further questions, please contact:
E-mail: workshops .microscopy .de @zeiss .com