Microscopy Events

ZEISS at Neuroscience 2016

November 12-16, 2016 - San Diego, CA, USA, Booth #1413

We look forward to seeing you at our largest North American tradeshow for life scientists. Test drive our newest technologies, including electron, confocal, zoom, stereo and compound microscopes. Grab a coffee on us, attend one of our daily seminars and learn how researchers are using ZEISS microscopes to push the limits of neuroscience. Celebrate the 200th birthday of Carl Zeiss with us and enter to win a replica of one of our original microscopes.

Ultraresolution Imaging

Imaging large, cleared samples

Better confocal imaging

Accomplish ultra-resolution imaging of neural connections and intracellular structures with electron microscopy. Learn about our solutions for 3D reconstruction of large volumes or find out about the fastest scanning electron microscope available – image up to centimeters of tissue.

With the only light sheet microscope on the market with lenses specific for cleared tissues and revolutionary Airyscan-equipped confocals, ZEISS is uniquely positioned to image large, cleared samples. Visit us at Neuroscience to see a Lightsheet Z.1 in action.
 

With Airyscan, and now with Fast technology, ZEISS confocals are revolutionizing imaging. With better signal-to-noise, superresolution with any fluorophore, and fast imaging, find out what you’re missing with your current confocal system. Test drive one at Neuroscience.

Image Credits

Middle Image: Image courtesy of Prof. Dr. Marc Spehr,  RWTH Aachen University, Germany.
Right Image: Image courtesy of Tong Xiao and Beth Carroll. Isacoff Labs. University of California, Berkeley.

 

200th Birthday

Celebrate the 200th birthday of our founding father, Carl Zeiss.

The mechanic Carl Zeiss opened a small workshop for precision mechanics and optics in Jena, Germany in 1846, laying the foundation for today’s global technology player ZEISS. His passion for precision is legendary and still characterizes ZEISS, its employees and its products to this very day.


Visit our booth to enter to win a replica of one of our original ZEISS microscopes.
 

 

Book A Demo

Test drive our equipment and learn how ZEISS can help you further your research.

 

Electron Microscopes
MultiSEM 505/506 Learn how you can unleash the acquisition speed of 91 parallel electron beams in the fastest scanning electron microscope in the world.
Book an appointment
GeminiSEM 500 FE-SEM Get sub-nanometer resolution and high detection efficiency, even in variable pressure mode. Book a demo
Confocal & 3D Imaging Systems
LSM 880 with Airyscan confocal microscope With the unparalleled combination of signal-to-noise, speed and resolution, experience the new standard in confocal imaging. Book a demo
LSM 800 with Airyscan
confocal microscope
Benefit from Airyscan technology in a confocal designed for individual labs. Book a demo
Lightsheet Z.1 light sheet imaging system Test drive how light sheet microscopy pushes the boundaries of 3D imaging of large samples. Book a demo
Cell Observer SD spinning disk microscope
Learn how spinning disk microscopy can image your live cells, gently, over long time periods. Book a demo
Automated Imaging Systems
Axio Scan.Z1 digital slide scanning system Increase your productivity. Load up Axio Scan.Z1 with up to 100 slides and image with a single push of a button. Book a demo
Research Microscope Platforms
Axio Examiner fixed stage microscope platform Designed specifically for examination and patch-clamp experiments on nerve cells and brain slices. Book a demo
Axio Imager 2 upright microscope platform From manual to fully motorized, configure specifically to your experimental needs. Book a demo
Axio Observer inverted microscope platform For imaging of either fixed samples or living cells, this inverted platform can serve a diverse set of imaging requirements. Book a demo
Axio Zoom.V16 zoom microscope platform A zoom microscope with outstanding resolution - try it for brightfield and fluorescence imaging of model organisms Book a demo

Join us daily at 12:00 pm for free coffee and learn from our customers as they share their data from some of our newest technology.

  • Agenda
    Date & Time Title
    Sunday, November 13, 2016
    12:00 pm
    Design and Application of New Voltage Sensitive Dyes for Ex vivo Brain Imaging
    Dr. Rishi Kulkarni
    Monday, November 14, 2016
    12:00 pm
    Correlative 3D In Vivo 2-Photon and Electron Microscopy Using Natural Landmarks
    Professor Dr. Jochen Herms
    Tuesday, November 15, 2016
    12:00 pm
    Next Generation Neuroscience: High Throughput Connectomics via MultiSEM Serial Array Tomography
    Alyssa Wilson
    Wednesday, November 16, 2016
    12:00 pm
    Expansion Microscopy with the ZEISS Lightsheet Z.1
    Dr. Shoh Asano
  • Abstracts

    Design and Application of New Voltage Sensitive Dyes for Ex vivo Brain Imaging

    November 13, 2016 12:00 pm

    Dr. Rishi Kulkarni, Miller Lab, University of California at Berkeley, Berkeley, California

    Fast changes in neuronal membrane potential drive their unique cellular physiology. Despite the biological importance of coordinated neuronal firing, observing activity in a non-invasive, highly parallel manner remains a challenge, due in part to a lack of tools that can report on fast changes in membrane potential with sufficient speed, sensitivity, and brightness. We develop a new design paradigm for xanthene-based voltage reporters based on a rigidized fluorophore that shows improved voltage sensitivity and a rhodol-based voltage reporter that demonstrates additional photostability and brightness under both one- and two-photon illumination. We use the new indicator, RhodolVoltageFluor-5, or RVF5, to probe dynamics of neuronal excitability in a mouse model of genetic epilepsy, both in culture and in brain slices.

     

     

     

    Correlative 3D In Vivo 2-Photon and Electron Microscopy Using Natural Landmarks

    November 14, 2016 12:00 pm

    Professor Dr. Jochen Herms, Ludwig-Maximilians-University, Munich, Germany

    In neuroscience research there is an increasing demand in correlative imaging techniques because of the complexity of the brain with morphological scales ranging from nanometers to several millimeters in the rodent brain. Our lab investigates the time course of synapse loss in mouse models of neurodegeneration like Alzheimer or Parkinson disease in relation to neuropathological hallmarks of the diseases. Furthermore, we are interested in the role of disease-relevant proteins in the structural plasticity of synapses, the ability of the brain to form and eliminate synapses for example in response to sensory inputs or a learning task. Structural plasticity can be measured by long-term in vivo 2-photon microscopy in combination with a mouse line that expresses a fluorescent protein (GFP or YFP) in a subset of neurons and their neuritic processes including dendritic spines. Even though these measurements give important kinetic insights into disease progression they miss the ultrastructural details and the context of the surrounding tissue. In the past 10 years several protocols were developed to correlate in vivo 2-photon with electron microscopy (EM) data based either on DAB-conversion of GFP or on near-infrared branding landmarks (NIRB). Here, we present a new workflow using natural landmarks that overcomes the difficulties with DAB-conversions and leaves the surrounding tissue next to the structure of interest intact in contrast to the NIRB-marks. The usability of the workflow is demonstrated by correlating the lifetime of dendritic spines with the ultrastructure of the synapses they establish. For the measurement of the lifetime of dendritic spines we applied in vivo 2-photon microscopy in combination with a cranial window implantation to assess the formation and elimination of single spines over time. After fixation of the mouse brain tissue the in vivo imaged regions are identified and mapped with their natural landmarks. The brain sections are stained for electron microscopy and embedded in resin. Finally, the in vivo imaged regions are localized in the EM surface images and 3D volumes of the dendrites are imaged using FIB-SEM. In order to confirm the identity of the dendritic segments 3D volume reconstructions were performed and correlated to the in vivo data.

     

     

     

    Next Generation Neuroscience: High Throughput Connectomics via MultiSEM Serial Array Tomography

    November 15, 2016 12:00 pm

    Alyssa Wilson, Harvard University, PhD Student, Cambridge, Massachusetts

    Recent developments in high-throughput, high-resolution imaging are now allowing for experimentation that was previously inaccessible due to the historical limitations of conventional electron microscopes. Serial array tomography studies for rapid 3D reconstruction of macroscopic volumes of mouse brain tissue, human retina, and entire C. elegans are presently underway using the innovative ZEISS MultiSEM technology which employs an array of imaging probes in parallel. Implications to neuronal and glial function, disease understanding, and statistical variability across populations in the context of connectomics will be elucidated from these results. Benefits of the 61-beam SEM instrument will be described based on the workflows established in the Lichtman Lab at Harvard University.

     

     

     

    Expansion Microscopy with the ZEISS Lightsheet Z.1

    November 16, 2016 12:00 pm

    Dr. Shoh Asano, Media Lab, Massachusetts Institute of Technology, Cambridge, Massachusetts

    In expansion microscopy (ExM), samples are embedded in a swellable hydrogel and then isotropically expanded by addition of water. This expansion allows samples to be imaged with a higher 'effective' resolution, while the almost complete optical clearing offers deeper imaging capabilities. ExM can be used with diffraction-limited optics and thus offers an attractive alternative to many superresolution techniques.

    However, with the resulting volumetric change of an expanded sample, high-speed and large-scale imaging methodologies are desired. Here we report how these demands are met with the ZEISS Lightsheet Z.1 system. Using 3D-printed adapters and other readily available tools, we take advantage of the stage design, which allows for imaging large, multicolor volumes of expanded brain slices.