Drosophila melanogaster embryo Jupiter-GFP (microtubules) ZEISS LSM 880 Airyscan, depth-coded projection Courtesy of Balazs Erdi, Max F. Perutz Laboratories, University of Vienna, Vienna Biocenter, Austria

Microscopy Course

Laser Scanning Microscopy

Advanced Course LSM 880

Date2018-09-26 - 2018-09-28
StatusRegistration: Expired
Registration deadline2018-09-16
LocationCarl Zeiss Microscopy GmbH, Carl-Zeiss-Promenade 10, 07745 Jena, Germany

For further information or questions, please contact: courses .microscopy .de @zeiss .com

Course Target
Besides their classical function as superior 2D and 3D imaging systems, advanced confocal laser scanning microscopes can be applied to study subcellular dynamics and to separate multiple fluorescent labels in a live cell environment. Confocal microscopy of living cells requires speed optimized imaging, minimized light induced lesions, and highest achievable resolution. If fluorescent labels with overlapping emission spectra are combined, simultaneous spectral acquisition followed by linear umixing can be used to separate them from each other. By using the Airyscan, you have the possibility to gain not only higher resolution but also higher sensitivity.

Master these innovative experimental approaches during this course and apply them successfully in your own research.

- Strategies for multiple fluorophore imaging
- Lambda stack acquisition, linear unmixing, and online fingerprinting
- Live cell imaging strategies
- Airyscan (in all modes: confocal, virtual pinhole, sensitivity, and superresolution imaging)
- Optimal Airyscan set-up (e.g. sample levelling or MBS adjustment)
- Operation of Airyscan Fast mode

All hands on will be exercised on a LSM 880 with Airyscan using the ZEISS ZEN software.

Fundamentals are recommended, e.g. our „Basic Course LSM 880“.

Course Fee: 2,709.00 EUR for a three day course excl. tax
Course material and lunch are included
(All prices are valid for business customers. If you are a private consumer we would be pleased to provide you with an individual quotation.)

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