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Next Generation Superresolution Microscopy with ZEISS Elyra 7

ZEISS Workshop

ZEISS Forum - Oberkochen

February 26 - 27, 2019


Take advantage of this unique opportunity to see the latest advances in superresolution microscopy live - with the recently launched ZEISS Elyra 7 featuring Lattice Structured Illumination Microscopy (SIM).

We are proud to welcome the 2014 Nobel Prize Laureate, Professor Eric Betzig, for a keynote lecture. Eric Betzig, who continues to pioneer research at the forefront of microscopy, will introduce new technologies and their application in multidimensional biological fluorescence imaging including superresolution microscopy.

Convince yourself at a second day of comprehensive workshop sessions to learn how your work can benefit from a flexible platform for fast and gentle superresolution imaging

  • Observe fast biological processes with an unprecedented speed of up to 255 frames / sec at superresolution using Lattice  
  • Reduce the light dosage on your precious specimen and still capture all the details – in multiple colors simultaneously
  • Resolve molecular structures in 3D with Single Molecule Localization Microscopy at up to 20 nm lateral resolution and unmatched 3D capture range of 1.4µm in one go
  • Use dual camera detection to simultaneously acquire two colors with high precision
  • Learn about the new Apotome mode for superfast optical sectioning with ZEISS Elyra 7

Don’t miss out – be one of the first to experience the next generation microscopy with ZEISS Elyra 7. 

ZEISS symposium and workshop are free of charge. Register for one of the few seats as soon as possible. Booking & expensis of accommodation are not included.

Details
Agenda
Registration
Hotel
Venue
February 26th, 2019 | Workshop

11.30 am

Bus Shuttle from Station Aalen to ZEISS Microscopy Customer Center Europe

12.00 am

Welcome & Lunch

1.00 pm

 

System Demonstrations Life Cell Imaging in 1 h Sessions:

  • Elyra 7 - Versatile Fast and Gentle 3D Superresolution
  • Elyra 7 - Application and Technology of the New Lattice Light Illumination
  • LSM 880 with Airyscan - Fast and Gentle Confocal Imaging 
  • Celldiscoverer 7 - Automated Live Cell Imaging
  • Lightsheet Z.1 - Multiview Imaging of Large Specimens with Light Sheet Fluorescence

6.45 pm

End

 

Bus Shuttle to Hotel Estilo
February 27th, 2019 | Pre-Program

9.00 am

Bus Shuttle from Hotel to ZEISS 

9.30 am

Optional: Factory Tour ZEISS Medical Solution Center

 

 

Bus Shuttle to ZEISS Forum

 

11.00 am

Visit ZEISS Museum of Optics | Relax & Work at Coffee Lounge ZEISS Forum

 
February 27th, 2019 | Symposium

12.00 am

Reception & Lunch

 

1.00 pm

Welcome

Dr. Markus Weber
President & CEO
ZEISS Research Microscopy Solutions

1.30 pm

Keynote Lecture:
Visualizing Intracellular Organelle and Cytoskeletal Interactions at Nanoscale Resolution on Millisecond Time Scales

Prof. Dr. Eric Betzig, Nobel Prize Laureate 2014 
Professor of Molecular and Cell Biology, University of California at Berkeley;
Eugene D. Commins Presidential Chair in Experimental Physics, University of California at Berkeley;
Investigator, Howard Hughes Medical Institute; Senior Fellow, Janelia Research Campus

2.30 pm

Superresolution Microscopy with Molecular Resolution

Prof. Dr. Markus Sauer
Department of Biotechnology and Biophysics Biocenter
University of Wuerzburg, Germany

3.20 pm

Revealing the Fastest Processes in Superresolution

Dr. Rebecca Elsässer
Business Sector Life Sciences
ZEISS Research Microscopy Solutions

4.05 pm

Coffee Break

 

4.45 pm

Factory Tours

  • Customer Center ZEISS Vison Care

 

 

Bus Shuttle to Explorhino Science Center

 

7.00 pm

Evening Event
Explorhino Science Center Aalen

 

 

Bus Shuttle to Suggested Hotels

 

For further questions please contact workshops .microscopy .de @zeiss .com

Download Invitation

Registration closed.

Suggested Hotel

Please book yourself before Feb 1 to guarantee availability and special ZEISS rate.
Quote the booking code “ZEISS Workshop” to get the ZEISS rate.

Only this hotel is served by the bus shuttle to all venues of the Workshop.

Design & Lifestyle Hotel Estilo 

Gartenstraße 30
73430 Aalen
Single Room 80 €

To book:
Tel. +49 7361780 78 0
est ilo @shotels .de

Venue February 26th

ZEISS Microscopy Customer Center Europe
Rudolf-Eber-Straße 2
73447 Oberkochen
Germany

Parking lots: reserved on the big parking lot at Rudolf-Eber-Straße.

Venue February 27th

ZEISS Forum
Carl-Zeiss-Straße 22
73447 Oberkochen
Germany

Parking lots: reserved on the parking lot in front of the ZEISS Forum.

Cells, widefield image (left) and Lattice SIM image (right)

Lattice SIM

Fast and gentle live cell imaging

Lattice SIM overcomes limitations of classic SIM by using an innovative spot illumination pattern instead of grid lines. The result is a breakthrough in light efficiency, enabling fast and gentle superresolution imaging of living specimen.

Microtubules - PALM (63x Oil)

Single Molecule Localization Microscopy

Faster, more flexibility, without compromising on resolution

With the efficient dual camera detection and high power laser lines across the visible spectrum, you're free to choose your dyes and markers for experiments.

Hippocampus with SIM
Hippocampus with SIM
COS-7 cells. Maximum intesity projection of 66 sections. Microtubules stained with Alexa 488 (green) and Actin stained with Alexa 568 (red). The Apotome mode allowed simultaneous dual ­color acquisition.
COS-7 cells. Maximum intesity projection of 66 sections.
SMLM: Alpha tubulin was labelled with Alexa 555 and beta ­tubulin with Alexa 488. The two channels were acquired ­simultaneously. The epitopes are either occupied by a green fluorophore or by a red one – shown by the mutual exclusion between the green and the red dye molecules.
SMLM: Alpha tubulin was labelled with Alexa 555 and beta ­tubulin with Alexa 488.