Expert course: Superresolution Microscopy Elyra 7

Superresolution microscopy enables insights into structures beyond the clas­sical resolution limit. Since the introduction of Elyra 7, structured illumination microscopy {SIM) has been brought to a new level. Groundbreaking light effi­ciency empowers gentle superresolution imaging with incredibly high speed: to examine the fastest processes in living samples - in large fields of view, in 3D, over long time periods, and with multiple colors.
Elyra 7 combines Lattice SIM with single molecule localization microscopy
{SMLM) for techniques such as PALM, dSTORM and PAINT.
This course will provide the skills to acquire Lattice SIM, PALM, and dSTORM data from
specimens and will enable you to perform the appropriate processing.

Topics of the course

 

Lattice SIM
  • Principle, acquisition and filtering
  • Versatility: SIM Mode, Leap Mode, Apotome Mode, Burst Mode
  • Camera alignment, channel alignment
  • PSF measurement
  • Processing
 
Single Molecule Localization Microscopy
  • Basics and applications
  • Comparison: SMLM is more than PALM
  • Multichannel imaging
  • Grouping and working with fiducials
  • Activation strategies
  • 3D PALM

 

Prerequisites

Basic knowledge of Light Microscopy

 

Date

13/01/2020 - 16/01/2020

 

Duration

4 day(s)

 

Language

English

 

Course Fees

Upon request

 

Location

Application Center, Jena,
Carl Zeiss Microscopy GmbH, Carl-Zeiss-Promenade 10, 07745 Jena, Germany
Your location upon request

 

Request Course

Venue

Training and Support Center Jena

Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany