From live imaging to cleared samples @ Lightsheet 7

After this course, you will understand the theory on light sheet microscopy, how to prepare a sample for light sheet imaging, acquisition of a light sheet sample, and how to process the acquired images.

Topics of the course

 

  • Beam path
  • Sample preparation and chamber building
  • Single side illumination and dual side illumination
  • Pivot scanning
  • Multiview quick setup and manual setup
  • Incubation
  • Dual side fusion and subsetting
  • Multiview processing: landmark, intensity, and interactive registration with fusion
  • Online processing
  • Deconvolution
  • Customer interfaces and system care

 

Prerequisites

Basic knowledge of Light Microscopy. High speed internet connection. Computer speakers or headphones, a reliable telephone connection. 

 

Date

March 1 - 5, 2021

 

Duration

5 x 4 hours

 

Language

English

 

Course Fees

3600 €

 

Location

Online

 

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Venue

Online