Superresolution Microscopy Elyra 7

Superresolution microscopy enables insights into structures beyond the clas­sical resolution limit. Since the introduction of Elyra 7, structured illumination microscopy {SIM) has been brought to a new level. Groundbreaking light effi­ciency empowers gentle superresolution imaging with incredibly high speed: to examine the fastest processes in living samples - in large fields of view, in 3D, over long time periods, and with multiple colors.
Elyra 7 combines Lattice SIM with single molecule localization microscopy {SMLM) for techniques such as PALM, dSTORM and PAINT.
This course will provide the skills to acquire Lattice SIM, PALM, and dSTORM data from specimens and will enable you to perform the appropriate processing.

Topics of the course


Lattice SIM
  • Principle, acquisition and filtering
  • Versatility: SIM Mode, Leap Mode, Apotome Mode, Burst Mode
  • Camera alignment, channel alignment
  • PSF measurement
  • Processing
Single Molecule Localization Microscopy
  • Basics and applications
  • Comparison: SMLM is more than PALM
  • Multichannel imaging
  • Grouping and working with fiducials
  • Activation strategies
  • 3D PALM



Basic knowledge of Light Microscopy



September 6 - 9, 2021



4 day(s)





Course Fees




Application Center, Jena,
Carl Zeiss Microscopy GmbH, Carl-Zeiss-Promenade 10, 07745 Jena, Germany
Your location upon request


Request Course


Training and Support Center Jena

Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany