Biology is the science that studies life. So it comes as no surprise that imaging of living organisms or cells has always been a central focus to drive research forward and provide unique insights. Being able to image living samples has allowed researchers to observe complex morphogenic processes such as the development of an organism over time, but it comes with its own challenges. Living samples are often delicate and finding the optimal imaging set up is critical for the success of the experiment. In this webinar we will discuss the imaging parameters that are important for your live imaging experiments and deduce the best imaging technique from them. We will look at a variety of different examples and evaluate which method would be the most suitable, leading to the best results. We will review imaging of samples from single cells in culture, all the way to imaging of larger 3D model organisms, such as zebrafish and cricket embryos. During the webinar we will talk about Widefield Microscopy with Deconvolution, Confocal Microscopy and Airyscan 2 Multiplex, Multiphoton microscopy, Structure Illumination Microscopy, and Lightsheet Microscopy. The individual strength of each method will be discussed in the context of live imaging, aiming to support you choosing the best instrument for your next experiment with living samples.