LSM 800 with Airyscan Airyscan now resolves 120 nm
ZEISS LSM 800 with Airyscan
Profit from High Productivity and Throughput

ZEISS LSM 800 with Airyscan

Your Compact Confocal for High-End Imaging

Dr. Ingo Bartholomäus, product manager, introducing LSM 800 with Airyscan.

Dr. Ingo Bartholomäus, product manager, introducing LSM 800 with Airyscan.

Confocal imaging demands the very best imaging quality. With LSM 800 you choose a flexible and compact confocal laser scanning microscope, complete with highly sensitive GaAsP detection and fast linear scanning. Add Airyscan, the revolutionary detection concept from ZEISS to acquire perfect optical sections with a resolution of 120 nm (in x,y) and 350 nm (in z) even in thick samples. With the 4 – 8× better signal-to-noise ratio (SNR) of Airyscan you will be pushing sensitivity and superresolution imaging beyond the limits of all conventional confocals.

LSM 800 is your entry into the world of high-end confocal imaging. Simply decide which options your system needs today, then upgrade in the future as your needs grow.

 

Highlights

Confocal_resize_Channel Alignment 2DSR_Channel

Capture smallest structures with Airyscan at 120 nm resolution
Confocal image (left)  / Airyscan image (right). Cells labelled with TOMM20 – Alexa Fluor 488 (green) and TIMM – Alexa Fluor 568

HeLa cells

HeLa cells;
Sample: courtesy of A. Seitz, EPFL, Lausanne, Switzerland

Perfectly Tailored to Your Needs

  • Use Airyscan to resolve 120 nm, combined with 4-8 x more sensitivity than any classic confocal
  • Use up to three highly sensitive GaAsP detectors
  • Perform fast linear scanning
  • Profit from high productivity and throughput
  • Benefit from great flexibility in live cell imaging
  • Get uncompromised image quality
  • Perform precise quantitative measurements
Python interface for ZEN imaging software

Convallaria, rare event detection with ZEN OAD.

Open Interfaces to Extend Your System

  • Get the full benefits of integrated incubation solutions and state-of-the-art Axiocams for your lab or multi-user facility
  • Intuitive ZEN imaging software for complex automated imaging routines with Experiment Designer
  • Easily exchange data with third party software
  • Define your own application world using the powerful open application development (OAD)
  • Use ZEISS Shuttle & Find for correlative microscopy and connect LSM 800 with your ZEISS electron microscope
Plant root (Arabidopsis thaliana)

Plant root (Arabidopsis thaliana);
Sample: courtesy of T. Pasternak, Institute of Biology, University Freiburg, Germany

Your Compact System for High-end Confocal Imaging

LSM 800 makes excellent economic sense:

  • Get an affordable system with an attractive price / performance ratio that is robust and easy to use
  • Save lab space and money: LSM 800 has a small footprint and minimal setup requirements, minimal maintenance and training, is self-calibrating and has low energy consumption
  • Enjoy predictable cost of ownership over the entire lifetime

Airyscan

Airyscan detector, schematic beam path

Airyscan detector, schematic beam path

Revolutionize Your Confocal Imaging with ZEISS Airyscan

Airyscan is a detector that draws on the fact that a fluorescence microscope will image a point-like source as an extended Airy disk (Airy pattern). In a standard confocal microscope the out-of-focus emission light is rejected at a pinhole, the size of which determines how much of the Airy pattern reaches the detector.

When you increasingly close the pinhole to reject out-of-focus light, you get a sharper image, but it’s also dimmer since a great deal of light is then lost. The smaller the pinhole, the higher the resolution, but – equally – the greater the loss in light. Airyscan solves this conundrum between resolution and light efficiency by imaging the Airy disk onto a concentrically-arranged hexagonal detector array. Its detection area consists of 32 single detector elements, each of which acts like a very small pinhole.

Isolated centrioles of Chlamydia;<br />   Sample: courtesy of P. Guichard, EPFL, Lausanne, Switzerland

Isolated centrioles of Chlamydia;
Sample: courtesy of P. Guichard, EPFL, Lausanne, Switzerland

The confocal pinhole itself remains open and doesn’t block light – thus all photons of the whole Airy disk are collected. The signals from all detector elements are then reassigned to their correct position, producing an image with increased signal-to-noise ratio and resolution.

An area detector consisting of multiple detector elements allows great flexibility in imaging modes. Instead of closing the pinhole and universally blocking light, each photon from the z-section is kept. The additional information about the Airy pattern gives access to a perfect optical section with highest sensitivity. Airyscan works with your standard samples and dyes. It's your choice, whether you use it to get better SNR, superresolution or speed.

Beam Path

LSM 800 beam path animation

LSM 800 beam path animation

A Streamlined Light Path with Surprising Flexibility

The compact light path of LSM 800 with Airyscan is designed for highest efficiency, with a minimum of optical elements for highest sensitivity. Fluorescence emission light travels through the main dichroic beam splitter with its outstanding laser suppression to deliver supreme contrast. Up to two patented variable beam splitter dichroics (VSDs) divert the respective spectral parts of the fluorescence emission light. You can freely define up to three detectors: multialkali, GaAsP or Airyscan.

Fast and Linear Scanning

HepG2 cells; FRET imaging with acceptor photobleaching

HepG2 cells; FRET imaging with acceptor photobleaching

Your Powerful Combination

LSM 800 gives you the benefit of increased scanning speeds so you can resolve those fast movements of labeled proteins that demand equally fast scanning. At an image size of 512 × 512 pixels you will be capturing events with up to 8 frames per second.

Your LSM 800 is constantly monitoring and calibrating the scanner position so you can count on a stable and even field of view with constant pixel times across the whole observation area. This patented linear scanning regime gives you a constant signal-to-noise level and uniform exposure by the illuminating laser throughout the scanned area, including your manipulated regions of interest.

With LSM 800 you will be using more than 80% of the scanning time for data acquisition. Signal-to noise improves by about 29% compared to sine scanning systems. Your experiments will always deliver quantitative data. Likewise, you can adapt the scan field at any time by panning or cropping it, and rotating it freely to best suit the geometry of your sample.

Application Videos

Drosophila brain<br />
Sample: courtesy of D. Reiff, Institute of Biology, University Freiburg, Germany

Drosophila brain
Sample: courtesy of D. Reiff, Institute of Biology, University Freiburg, Germany

Mouse retina. Mueller cells stained for RFP (red), Amacrine cells stained for Chat (green), Cone photo receptors stained for mCar (white) and DNA stained with Hoechst (blue);<br />
Sample: courtesy of B. Roska, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland

Mouse retina. Mueller cells stained for RFP (red), Amacrine cells stained for Chat (green), Cone photo receptors stained for mCar (white) and DNA stained with Hoechst (blue);
Sample: courtesy of B. Roska, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland

Division of LLC-PK1 (pig kidney epithelial) cells, green: tubulin-GFP, red: H2B-mCherry.

Division of LLC-PK1 (pig kidney epithelial) cells, green: tubulin-GFP, red: H2B-mCherry.

Drosophila; neuromuscular junction stained for Bruchpilot (BRP).<br />
Sample: courtesy of J. Pielage, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.

Drosophila; neuromuscular junction stained for Bruchpilot (BRP).
Sample: courtesy of J. Pielage, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.

Drosophila; neuromuscular junction stained for Bruchpilot (BRP).<br />
Sample: courtesy of J. Pielage, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.

Drosophila; neuromuscular junction stained for Bruchpilot (BRP).
Sample: courtesy of J. Pielage, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.

Drosophila; neuromuscular junction stained for Bruchpilot (BRP).<br />
Sample: courtesy of J. Pielage, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
Mouse brain slice

Sample: courtesy of P. Janz, Neuro- pathology, University Freiburg, Germany

Mouse brain slice<br />
Sample: courtesy of P. Janz, Neuro- pathology, University Freiburg, Germany

Mouse brain slice
Sample: courtesy of P. Janz, Neuro- pathology, University Freiburg, Germany

Mouse brain slice<br />
Sample: courtesy of P. Janz, Neuro- pathology, University Freiburg, Germany
Mouse brain slice

Sample: courtesy of P. Janz, Neuro- pathology, University Freiburg, Germany

Downloads

ZEISS LSM 800 with Airyscan

Your Compact Confocal for High-End Imaging

Pages: 27
Filesize: 7,893 kB

White Paper: Airyscanning - Short version

A Novel Approach to Confocal Imaging

Pages: 5
Filesize: 1,709 kB

Beampath - ZEISS LSM 800 with Airyscan

Your Compact Confocal Power Pack

Pages: 2
Filesize: 2,628 kB

Technology Note: The Airyscan Detector from ZEISS

Confocal Imaging with Improved Signal-to-Noise Ratio and Superresolution

Pages: 19
Filesize: 3,958 kB

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